米曲霉单宁酶产酶条件及其应用的研究-微生物学专业毕业论文.docxVIP

朱曲霉单宁U-产茸●条件反：R-．alZ．J■I的研完酶促反应最适温度为42C，最适pH值为6．5。同时，该酶在50。C以下，pH 朱曲霉单宁U-产茸●条件反：R-．alZ．J■I的研完 酶促反应最适温度为42C，最适pH值为6．5。同时，该酶在50。C以下，pH 3．o_7．5范围内均比较稳定。 以壳聚糖作为载体，戊二醛为交联剂，采用交联的方法将单宁酶固定。 结果表明， 酶活力固定率为73．86％；固定化酶的最适酶促反应温度为50 ℃，比游离单宁酶提高8。C；最适反应pH值为6．0，比游离的向酸性漂移 了0．5个单位；对热和pH的稳定性有所提高；对底物的亲和力下降；以没 食子酸丙酉旨作为底物时，固定化单宁酶的半衰期为22．68h。 关键词：单宁酶产酶条件酶学性质固定化茶乳酪 Ⅱ 广西大掌硕士掌位论文 广西大掌硕士掌位论文 朱曲霉单宁嗣}产酶条件及|}五L用的研究 STUDIES 0N PRODUCTION CONDITIONS A ND APUCAnON OF删NASE FROM Aspergillus oryzae ABSTRACT Tannase can cleave the ester bonds and depside bonds of tannin acid， producing gallic acid and glucose，and the ester linkages of the compound in cold tea．Tannase is widely used in tea beverage，beer process，feedstuff, cosmetic，leather industry and SO on．currently,the cost of producing tannase is very high，due to the low yield of tannase from the current microorganism strains and the non—optimal conditions for producing tannase．To reduce the production cost and make it to be utilized rapidly in industry,it is meaningful to study the techniques on how to induce high yield of tannase from microorganism． The conditions for tannase production from Asperyillus Oryzae was studied． It shows that Revulsant 2 can also induce mycelium to produce tannase，besides Revulsant 1(tannic acid)．In this study,we use a new idea which has not been reported in our country to induce Asperyillus oryzae to produce tannase．That is， eugonic mycelium was first gained on nutrition—rich medium．Then eugonic Ⅲ g-．西大学硕士掌位论文 g-．西大学硕士掌位论文 米曲霉单宁§■产萄I条件a其应用的研究 mycelium was induced to produce tannase on inducement medium containing Revulsant 2．The activity of tannase acquired at this condition is 2．5 times higher than that acquired on the nutrition—rich medium containing Revulsant 2．The optimal inducement condition of this method is that inducement culture medium contains 1．5％Revulsant 2，initial pH is 5．0，culture for 72h under 30。C．The activity of tannase acquired at the optimal inducement condition reach up to 1