毛冬青酸预处理对家兔肝缺血再灌注损伤时肿瘤坏死因子的变化及对肝脏的保护作用-外科学专业毕业论文.docxVIP

硕士学位论文 硕士学位论文 英文摘要 ABSTRACT Objective：To explore the Effects of Lexolic preconditioning on the TNF—a and protection in hepatic ischemia—reperfusion in rabbits Methods：30 rabbits were randomly divided into 3 groups：normal control group(Group A)，ischemia-reperfusion 2hours group(Group B) and pretreated the ischemia—reperfusion with Ilexolic group(Group C)．10 rats each group． The ischemia-reperfusion model was produced by Pringle’S method． It was produced by claming the hepatic artery and portal vein with a clamp for 30 minutes．then reperfusing 2 hours．The hepatic tissues and blood samples were collected 30 minutes after ischemia and 2 hours after reperfusion in the B，C groups．Then the activity of MDA；the concentration of TNF-Q，ALT and AST were measured and hepatic tissue have the pathological examination． RESUITS 1．Compared to Group A，the劁LT，AST,the activity of MDA，the n盯-Q all increased in B，C．And the injury of liver was found by pathological examination． 2．Compared to Group B，the medicine of C produced predominant effective(P0．05)． CONCLUSloN 硕士学位论文 硕士学位论文 英文摘要 ILa could reduce the TI师一Q，decreasing the concentration of oxygen free radical．Improving the energy metabolism of the ischemic liver tissue，prevent and reduce the hepatic ischemia reperfusion injury KEY WORDS ILa，hepatic，ischemic reperfusion injury，TNF— Q III 硕士学位论文 硕士学位论文 缩略词一览表 缩略词一览表 英文缩写 英文全称 中文名称 Ilcx pubcscens Hook．et Arm． 毛冬青 II_a flexoHc acid 毛冬青酸 I／R Ischemia Reperfusion 缺血再灌注 E『】卜1 Endothelm．1 内皮素一1 IRI Ischemia-Reperfusion Injury 缺血再灌注损伤 NO Nitric Oxide 一氧化氮 IL广8 Interleukin．8 白细胞介素一8 TNF-Q Tumor Necrosis Factor-a 肿瘤坏死因子．a PAF Platelet Activating Factor 血小板活化因子 KC Kupffer Cell 枯否细胞 PMN Polymorphonuclear Leukocyte 中性粒细胞 TXA2 Thrombxane A2 血栓素A2 NK-B NudearfacterK-B 核因子K．B MDA mal∞dialdehyde 丙二醛 PGT2 Prostacyclin 12 前列环素12 IV 原创性声明本人声明，所呈交的学位论文是本人在导师指导下进行的研究工作及取得的 原创性声明 本人声明，所呈交的学位论文是本人在导师指导下进行的研究工作及取得的 研究成果。尽我所知，除了论文中特别加以标注和致谢的地方外，论文中不包含 其他人已经发表或撰写过的研究成果，也不包含为获得中南大学或其他单位的学 位或证书而使用过的材料。与我共同工作的同志对