抗癌中草药及其与DNA相互作用的电化学研究-分析化学专业毕业论文.docxVIP

摘要 摘要 王立萍 Abstract Currently,herbal medicines especially anticancer herbal drugs are gaining more attention from modem pl协m蛆ccIltical institutes,as scientists become羽黼nl砒herbal medicine is柚almost infinite resourt∞for drug development．Furthermore,the toxicity of the herbal drug is very low and most of them have no side-effect．The exploitation of the natural souree makes the diffence in the field of the medicine．As me known,the study ofherbal drugs mainly embody the methods ofpurification and separation,it is not enouO to study吐Ie ch棚疆cl廿s and nle anticancer mechanism of them．This dissertation have investigated the electrochemical d[Ia峨∞tcfs of emodin and apigenin and established the novel electroanalyfical methods for thenL Moreover,the interactions of emedin and apigenin with DNA arc then investigated by the eJ翻打∞h釉is灯y and spoc咖m．The result indicates emodin啪intercalate into the double helical slructure of DNA to inhibit the synthesis of DNA．Apigenin do∞ll’t interact with DNA under the coeditinn-％This simultaneously exhibits the low toxic effect of apigenin tocertain extent．The main works and results帅 summerized撼following： 1．The electrochemical behavior ofemodin矗the Oa-鹞Y carbon electrode has been investigated．In O．05 M NH3-NH4C!(50％ethanol，pH 7．2)buffer solution,a p曩if of quasi-reversible redox peaks砒potentials of 点pl=-0．688Vand岛2=-0．628Vandoneirreversible anodic peak啦旆3=-0．235Vwereappeared．They areall adsorption-driven p髓l【s．The peak currents of P3 ere linear relationship with apigenin concentrations in the rangeof8．9x10-8mol／L～7．8x10-6mol／Lwiththedetectionlimitof7．8x10．9mol／L．Basedonthismethod，the apigenin content of Sanbuang tablets was determined with satisfactory．There is need to separate other 9’ 10-anthraquinone derivative ingredients contained in herbal drug． 2．The electrochemical behavior ofthe anfitomor herbal drug apigenin was studied in 0．1 M B-R buffer solution(50％ethanol,pH 9．O)by mea；∞of some electrochemistry methods on the glessy carbon electrode