黄精多糖对老年糖尿病鼠糖基化终产物受体mRNA表达的影响-老年医学专业毕业论文.docxVIP

硕士学位论文 硕士学位论文 中文摘要 摘要 目的：探讨黄精多糖对老年糖尿病小鼠脑组织糖基化终产物受体 mR．NA(RAGE mRNA)表达的调节作用。 方法：BAIB，C健康小鼠30只，按随机数目表法分为对照组、 糖尿病模型组、治疗组，每组10只。糖尿病模型组和治疗组注射链 脲佐菌素建立小鼠糖尿病模型，治疗组给予黄精多糖2 mg／kg每日1 次灌胃给药，共12周，采用RT-PCR法测定各组实验动物脑组织 RAGE mRNA的表达。 结果：①糖尿病模型组实验鼠脑组织RAGE mRNA表达增加， RAGE／t3一actin相对值(0．153+_0．054)明显高于对照组(0，P0．01)。 ②应用黄精多糖治疗后，治疗组鼠脑组织RAGE mRNA表达较 模型组降低，RAGE／B—actin相对值∞．0924-0．033)显著低于模型组 ∞．153±O．054，P0．05)。 结论：黄精多糖能抑制老年糖尿病小鼠脑组织RAGE mRNA表 达，对高血糖及糖基化终产物(AGE)造成的脑组织损伤具有保护作 用。 关键词：糖尿病，实验性，糖基化终产物受体，黄精，多糖类 硕士学位论文 硕士学位论文 英文摘要 Objective：To study the regulative effect of Polygona-polysaccharose on the mRNA expression of RAGE(receptor of advanced glycosylation end products)in brain of aged diabetic rats． Methods：Thirty BALB／C healthy rats were divided randomly into control group，diabetic model group and treated group by random number labering method．Each group included ten rats．Rats in diabetic model group and treated group were injected with streptozotocin in its abdominal cavity Rats in treated group were given polygona-polysaccharose(dose in 2 ml／kg，once a day)by gastrogavage for twelve weeks．RT-PCR method was used to examine the expression of RAGE mRNA in rat brain． Results：(童)The expression of RAGE mRNA of brain tissue in aged diabetic rats was increased．The value of RAGE／13一actin(o．153±0．054) in aged diatetic rats was significantly higher than that in the control group 佃0．01)． 厘)After the treatment of polygona—polysaccharose the expression of RAGE mRNA in brain tissue WaS decreased in the treated group rats．The AGE／13一actin value in the treated group r0．092±0．033)was significantly lower than that in the diabetic model group伊0．05) Conclusions：Polygona·polysacchrose may decrease the expression Ⅱ 硕士学位论文 硕士学位论文 英文摘要 of RAGE mRNA in the tissue of brain in aged diabetic rats and has protective effect agaist the tissue damage caused by hyperglycemia and advanced glycolation end products． KEY WORDS：diabetes mellitus experimental， receptor of advanced glycosylation end products，Polygons