马鞭草的活性成分研究-药物化学专业毕业论文.docxVIP

其中 MBC-1, 2, 8, 11, 17, 21, 24, 25, 26, 27 为首次从该植物中分到,MBC-15, MBC-19 为新化合物，MBC-15 是通过四圆环和过氧桥连接的二聚体，这在自然 界中首次被发现，结构新颖；MBC-19 则与一般的环烯醚萜类不同，双键的位置 发生改变，这类环烯醚萜苷在自然界中也比较少见。 本课题研究了 13 个单体化合物对 RAW264.7 巨噬细胞 NO 代谢的影响，结 果显示化合物 MBC-12 对 RAW264.7 巨噬细胞产生 NO 有一定的抑制作用。 关键词：马鞭草，环烯醚萜苷类，三萜类，NO 活性 STUDIES ON THE ACTIVE CONSTITUENTS OF VERBENA OFFICINALIS L. ABSTRACT Verbena officinalis L. (Verbenaceae), a perennial plant grows wild throughout all temperate regions of the world. This herb has been used as folk medicine for thousands of years for treating acute dysentery, enteritis, amenorrhea and bronchitis. Previous phytochemical studies on this plant mostly focused on triterpenes, flavonoids, iridoids and phenylethanoid glycosides. In this dissertation, we investigate the chemical constituents from the aerial parts of V. officinalis L. which was collected from Huaxi Gaopoxiang, Guizhou Province in China. Thirty-three compounds were isolated from the aerial parts of V. officinalis L. by silica gel, sephadex LH-20 and preparative HPLC and thirty compounds were identified as follows by spectral analysis: MBC-1: Tridecane MBC-2: 5α, 8α-Epidioxyergosta-6, 22-dien-3β-ol MBC-3: β-Sitosterol MBC-4: Oleanoic acid MBC-5a: 3-Eepioleanoic acid MBC-5b: 3-Epiursolic acid MBC-6: 3α, 24-Dihydroxy-urs-12-en-28-oic acid MBC-7: Apigenin MBC-8: 1-Hydroxyl-tetracontane MBC-9: Ursolic acid MBC-10: Daucosterol MBC-11: Barbinervic acid MBC-12: Luteolin MBC-13: 4-Hydroxywogonin MBC-14: Verbenalin *MBC-15: 3-Ethoxy-6-carbonyl-phlomurin MBC-16: 3, 4-Dihydroverbenal MBC-17: Martinoside MBC-18: Quercetin *MBC-19: Verbean A MBC-20: 3α, 24-Dihydroxy-olean-12-en-28-oic acid MBC-21: 4-Dodecyl-1, 2-benzenediol MBC-22: Luteolin-7-O-β-glucoside MBC-23: Hastatoside MBC-24: SaussureosideB MBC-25: 2-(3, 4-dihydroxy)-phenylethanol MBC-26: Cistanoside E MBC-27: Castanoside A MBC-28: Isoverbascoside MBC-29: Acteoside Among them, MBC-15, 19 were new compounds, MBC-1, 2, 8, 11, 17, 21, 24, 25, 26, 27 were isolated from this