美满霉素对癫痫大鼠海马神经元凋亡的影响-人体解剖与组织胚胎学专业毕业论文.docxVIP

华 华 中 科 技 大 学 硕 士 学 位 论 文 PAGE 2 PAGE 2 Ⅱ～Ⅲ级。免疫组织化学方法结果显示：在 KA 致痫后 2h Cyt C 即开始有阳性 表达，8h 达到高峰，24h 表达减少，而 MC 预处理明显减弱此效应。RT-PCR 和免疫组织化学方法结果显示 caspase-3 mRNA 的含量及 caspase-3 免疫反应性 在 24h 时间点三组之间无明显差异，在 48h 时间点，KA 组明显高于对照组 (P0.05)，MC 预处理则明显拮抗 KA 诱导的 caspase-3 高表达。Western Blot 结 果显示：大鼠海马组织 Bcl-2 和 Bax 蛋白的表达水平呈负相关。与对照 NS 组相 比，KA 组 Bcl-2 蛋白表达降低而 Bax 蛋白表达升高；与 KA 组相比，在 MC+KA 组 Bcl-2 蛋白表达升高而 Bax 蛋白表达降低。且 Bcl-2/Bax 值在 48h 时间点比 24h 时间点更低。 上述研究结果提示，KA 致痫能诱导大鼠海马神经元凋亡，而 MC 预处理 不仅能抑制 KA 诱导的致痫效应，而且能减少 Cyt C 从线粒体释放入胞浆，从 而减少凋亡体的生成；并抑制凋亡效应蛋白 caspase-3 的激活，抑制细胞凋亡的 发生；另外还通过增大 Bcl-2/Bax 的比值抑制凋亡发生，这些结果提示 MC 对 KA 致痫大鼠海马神经元发挥了抗凋亡的神经保护作用。这对于临床癫痫防治、 神经保护有重要的启示意义。 关键词：癫痫；海马神经元；美满霉素； 凋亡； 海人酸 the Effection of Minocycline to Apoptosis of Hippocampal Neurons on Epilepsic Rats Master Candidate：YAO Qing Supervisor：Prof. LI Zheng-li Abstract Epilepsy is a common chronic disease in the nervous system. Due to the complexity of its pathogenesis and the diversity of its types, it is still a key issue to overcome the prophylaxis and treatment on epilepsy for the nerve scientists. Seizures can cause a variety of apoptosis-related gene expression and encephalo-neuron apoptosis, and this apoptosis in hippocampal is the most significant. This may be one of the reasons in leading to cognitive dysfunction and the later’s affective disorder on patients of epilepsy. Some studies show that minocycline (Minocycline, MC),the second-generation semi-synthetic tetracycline derivative, has neuroprotective effects in central nervous system injury and neurodegenerative diseases on animal models. These reports also think that its beneficial effects are correlated with its reducing nerve inflammation and obstructing cell death. However, its research on epilepsy is still relatively small. So, this study was to investigate the protective effect of minocycline on apoptosis of hippocampal neurons in epilepsic rats and to provide some informations for the clinical treatment of epilepsy. 96 adult SD male rats were used in this st