毛细管电泳激光诱导荧光检测法分析拟南芥中的茉莉酸-生物化学与分子生物学专业毕业论文.docxVIP

华 中 华 中 科 技 大 学 硕 士 学 位 论 文 II II 荧光检测法的理想。毛细管电泳激光诱导荧光检测(LIF)具有最高的灵敏度。目前,高 效毛细管电泳激光诱导荧光检测法已经广泛用于生物和临床分析，这种高灵敏度的分 析方法还没有用于对茉莉酸的检测分析。 本论文的主要研究内容是建立一种以毛细管电泳激光诱导荧光检测（CE-LIF）为 基础,以 8-氨基芘-1，3，6-三磺酸三钠盐（APTS）为衍生试剂的新方法,对拟南芥叶 片中的茉莉酸分离检测。 关键词：毛细管电泳；激光诱导荧光反应；茉莉酸；8-氨基芘-1，3，6-三磺酸三钠； 拟南芥 PAGE III PAGE III Abstract Jasmonic acid (JA), a linolenic acid-derived cyclopentanone, is a new kind of plant hormone. It plays regulatory roles in plant development and responses to environmental stresses, such as mechanical wounding, fungal infection, insects and microbial pathogen attack, drought and osmotic stress. In addition, there have been numerous reports on the physiological effects of jasmonates, including the inhibition of seed and pollen germination, stem growth, cell expansion and multiplication and the promotion of senescence abscission and tuber formation . Moreover, recent studies showed that JA could regulate the expression of many genes involved in plant development or in stress tolerance . In general, the concentration of JA in plant tissues is very low, as those of the other plant hormones. The quantification of endogenous JA level in plant extracts by means of a sensitive and reliable method is of interest because of the significance of these compounds as putative signaling molecules. Knowledge about establishing a higher sensitive analysis method is of great importance for understanding the physiological functions of JA. At present, several methods for determining the exist and concentration of JA have been reported, such as mass spectrometry (MS) , gas–liquid chromatography– mass spectrometry (GC–MS) , enzyme linked immunosorbent assay, high performance liquid chromatography (HPLC), radioimmunoassay (RIA) . However, the procedures of both MS and GC–MS method, which required long chromatographic separations at elevated temperatures, could cause thermal decomposition of the analytes. As a result, JA could not be discriminated perfectly from other plant components, whi