山豆根种子内生真菌及其抑菌活性成分研究-植物学专业毕业论文.docxVIP

出6个单体化合物，分别是大黄素、coniochaetones 出6个单体化合物，分别是大黄素、coniochaetones B、penicitrinol A，麦角 甾．5，7，22．三烯．3p．醇、肉豆蔻酸和软脂酸。 关键词：山豆根 内生真菌发酵产物结构鉴定 万方数据 Research Research on the Antagonistic Phytopathogen C onstituent of Endophytic Fungi from the Seeds of Sophora Tonkinensis ABSTRACT The root and rhizome of Sophora tonkinensis Gagnep．have high value of medicinal function such as heat-clearing，detoxicating，detumescence and relieving sore throat，owing to the main chemical composition of alkaloids， flavonoids and polysaccharide contmned in it．In previous studies，it has been shown that matrine and oxymatrine are the biological activity constituent in S． tonkinensis，such as antibacterial activity in vitro，antiviral，antitumor, antioxidant and enhance immune activity． The quantity and kind of endophytic fungi from medicinal plants are big and variety．Many new structure of compounds that most have the unique biological activity were extracted from endophytic fungi by academics．In recent years，endophyte，as a new kind of microbial resources，has attracted a great deal of aRention．To find and discover new active compounds from endophytes is a hotspot in the research on endophytes．At present，the information on tonkinensis endophytic fungi study has not yet to be seen at domestic and overseas，and the study is almost in a blank state．Based on this，the isolates of endophytic fungi from the seeds of S．tonkinensis were preliminary studied，and the main results were as follows： 万方数据 (1)A (1)A total of 28 endophytic fungi were isolated from tissue segments of＆ tonkinensis seeds with the method of peeling off the skin and disk fragment methodology．The isolates belong to 1 4 species of two classes Deuteromycetes and Ascomycota．Colletotrichum aft．boninense LA79，Colletotrichum graminicola，Colletotrichum hymenocallidis and Diaporthe pseudomangiferae are the dominant species and the isolation percentages are respectively 1 7．86％， 14．29％，10．71％and 10．71％． (2)The inhibition ratio，which was about the is