少突胶质细胞在体外培养中的发育和髓鞘形成-人体解剖学专业论文.docxVIP

少突胶质细胞在体外培养中的发育和髓鞘形成中文摘要 少突胶质细胞在体外培养中的发育和髓鞘形成 中文摘要 少突胶质细胞是中枢神经的成髓鞘细胞，对神经元轴突的快速电传导等起着重 要作用。少突胶质细胞的发育主要分为少突胶质前体细胞(具有增殖能力)和少突 胶质细胞(具有髓鞘形成能力的分裂终期细胞)。成年时的少突胶质前体细胞在在 一定条件下具备神经干细胞特性。 ＼本实验对少突胶质前体细胞进行研究的主要结果： 1．新生大鼠大脑皮质分散培养出现胶质细胞分层后，振荡分离少突胶质前体细胞 (大于95％)和星形胶质细胞。 2利用B104神经母细胞瘤条件培养液培养视神经外植块，可以使视神经外植块中的 少突胶质前体细胞选择性迁出(大于95％)。 3将种植视神经外植块的盖玻片与星形胶质细胞联合培养，少突胶质细胞从外植块 中迁出并发育，其细胞类型比例可能体现在体发育情况。 4从视神经外植块中迁出的少突胶质细胞在聚酯纤维上迁移，并发出纤细突起反复 缠绕非轴突纤维，可能模拟中枢神经髓鞘形成的起始和行为。 5．纯化的大鼠少突胶质前体细胞与小鼠小脑外植片(阿糖胞苷预处理)联合培养， 髓鞘和髓磷脂样结构出现在神经元轴突周围。 6．剥夺培养液中的氧和／或葡萄糖，检测乳酸脱氢酶释放量、流式细胞分析以及形态 学观察发现，少突胶质前体细胞对缺氧的耐受力大于对缺糖／缺血的耐受力。 结果提示，大鼠少突胶质细胞系细胞在体外培养条件下具有增殖、分化成熟和 髓鞘化能力等在体发育特性。』 ／ 义 关键词：少突胶质前体细胞；少突胶质细胞；发育；髓鞘形成；视神经外植块； ∥台104神经母细胞瘤条件培养液；体外培养：新生大鼠 中图分类害、／Q21，Q249，Q256 Development Development and Myelination of Oligodendrocyte／n Vitro Abstract Oligodendrocytes，the myelin-forming cells ofthe central nervous system，support neuronal processes for its saltatory conduction．So far，the causes of demyelinated axons in the CNS are beyond understanding．111e development of oligodendroglial lineage cells is continuous． but Call be chiefly divided into proliferating precursors and postmitotic oligodendrocytes (myelinating properties)．The adult oligodendrocyte precursor cells are of similar characteristies in some extent to the neural stern cells． The present results based on oligodendrocyte precursor cells include： 1．The primary glial cells from neonatal rat cerebral tissue were cultured 7-9 d．i V．The oligodendrocyte precursor cells and astrocytes were separated by orbital shaker at the onset of the stratification of glial ceils．The preparation of oligodendrocyte lineage cells were95％． 2．The oligodendrocyte prcursor ceils removed selectively from the explants of optic nerve exposed to the neuroblastoma B104 conditioned medium． 3 The oligodendrogUal lineage cells removed rapidly from the explants of optic nerve over the confluent aslTocytes．ne occulTence ofvarious oligodendroglial types may represent the development ofoligodendrocytes in vivo．