利用猪血制备氯化血红素技术的研究-食品科学专业论文.docxVIP

AbstractHeme，the Abstract Heme，the prosthetic group of the hemoglobin，has been used widely and importantly．Oxiding converts heme to hemin when separating heme and globin， so extracting heme from porcine blood is generally preparing hemin．There are two aim for preparing hemin from porcine blood．firstly，it develop full utilization Bf porcine blood，secondly，it can provide lots of hemin．On the base of analysis the dominance of methods for preparing hemin on trial，a new method was created，that is the whole technological chain of preparing hemin was separated into three steps：comminutingRBC，separating heme and globin from Sb，extracting hemin，the optimum technology and correlated theories in every step were searched．There were conclusions as follows， A：The best method for comminuting RBC is comminuted RBC by using reagent， the optimum conditions for comminutingRBC were reaction containing chloro— rm and blood corpusc]_e at a 0．3：l(V：V)ratio，ethanol and blood corpuscle at a 0．2 5：1(V：V)ratio，and distilled water and blood corpuscle at a 1．5：1 fV：V)raLio for 3 0 railrote stirring． B：Citric is as separat Jng agent-the optimum eonditions for aci．d separating techniques were reaction solution containing acetone and blood corpuscle at 6：1(V：V)ratio，1M sodium chloride and blood corpuscle at a 7 mill ilitres：20 millilitres(V：V)ratio at pH 2．80 foI 8 hours C：CMC—Na is as extracting agent of heme．the optimum conditions for the extracting techniques of heme were reaction solution containJ ng CMC—Na and blood corpuscle at a 1．59：i00 millilitres ratio at pH 6．00 for 8 hours．Results show that the content of hemin was about 5．16％，total output of pure hemin was 95．img／lOOmlPorcine blood，yield of hemin was 31．3％ Key words：Porcine blood Hemin Comminuting Separating Extracting 独创性声明本人声明所呈交的论文是我个人在导师指导下进行的研究工作及取得的研 独创性声明 本人声明所呈交的论文是我个人在导师指导下进行的研究工作及取得的研 究成果。尽我所知，除了文中特别加以标注和致谢的地方外，论文中不包含其他 人已经发表或撰写过的研究成果，也不包含为获得湖南农业大学或其它教育机构 的学位或证书而使用过的材料。与我一同工作的同志对本研究所做的任何贡献均 已在论文中作了明确的说明并表示了谢意。 研究