临床分离的铜绿假单胞菌对头孢菌素类抗生素耐药机制的研究-药理学专业论文.docxVIP

四川大学硕七学位论文CTX．M型ESBLs的菌株。他唑巴坦抑制ESBLs的作用强于克拉维酸和舒 四川大学硕七学位论文 CTX．M型ESBLs的菌株。他唑巴坦抑制ESBLs的作用强于克拉维酸和舒 ● 巴坦，而苯唑西林对ampC酶的抑制作用较弱。携带有质粒介导的DHA．1 型AmpC酶基因的感受态细胞E．coli DH5a水解Nitroeefin法阳性。合用 CCCP后有4株的MIC值较单用头孢噻肟的MIC值下降了至少2个梯度， 且这4株菌的MexAB．OprM的表达量较其余菌株高。 结论：临床分离的62株铜绿假单胞菌对亚胺培南、美洛培南、头孢吡 肟、头孢他啶、头孢哌酮、头孢噻肟、头孢曲松的耐药率较高；产B．内酰 胺酶(ESBLs和AmpC酶)和主动外排作用是这临床分离的62株铜绿假 单胞菌对头孢菌素类抗生素耐药的两种机制。其中产ESBLs主要以TEM 型为主，酶抑制剂以他唑巴坦的作用较强，质粒介导的B．内酰胺酶基因具 有转移性。 ● 关键词：铜绿假单胞菌；耐药机制；B．内酰胺酶；主动外排作用 ● 四川大学硕十学位论文■ 四川大学硕十学位论文 ■ Study on the resistance mechanisms of cephaIosporins—resistant to clinical isolated of Pseudomonas aerugmosa Graduate student：Xie Fen Tutor： Zhou Liming Abstract objectives：To stIldy the resistance ofPseudomonas aeruginosa to several 13-1actum amibiotics and the resistance mechanisms of cephalosporins．resistant to clinic isolated ofPseudomonas aeruginosa． Methods：The susceptibility testing was performed with agar dilution test； hydrolyzing Nitrocefm method to testify IBqactamases；screening and confirmatory test for extended-spectrum 13-1actamases (ESBLs) and three-dimensional test for AmpC enzyme；DNA was sequenced by PCR amplification to identify the type of IB-lactamases；modified three-dimensional test was used to evaluate the effect of p-lactamase inhibitors(clavulanic acid, sulbactam,tazobactara，EDTA and oxacillin)；transformation to testify the transfer of IB-lactamase gene；the experiment of CCCP inhibition and leVerse ● transcdption-polymerase chain reaction(RT-PCR)were used to deteete active efflux pump． Results：The resistant rates to imipenem、melopenem、cefepime、 ceftazidime、cefoperazone、cefotaxime and ceflriaxone were 45．16％、29．03％、 27．42％、30．64％、58．06％、58．06％and 67．64％in the 62 strains of 4 四川大学硕七学臂论文Pseudomonas 四川大学硕七学臂论文 Pseudomonas aeruginosa from Hospital in Chengdu,respectively．The 24 ■ strains produced 13-1actamases including the 3 strains producing ESBLs and the 3 strains overproducing AmpC eflzyme in the phenotypic tests．The 19 strains by PCR amplification produced TEM ty