六味地黄丸对大鼠2型糖尿病周围神经病变的干预及机制分析-药理学专业论文.docxVIP

减少，纤维分布稀疏，神经髓鞘细胞有变性肿胀、坏死缺失，髓鞘板层与 神经纤维分离现象显著，有脱髓鞘现象。各药物干预组与糖尿病模型组比 较，纤维分布较密，脱髓鞘现象较轻。 结论：1 喂养大鼠高糖高脂饮食并低剂量注射STZ，可诱导出现胰岛素抵抗 2型糖尿病模型。 2 六味地黄丸对2型糖尿病神经组织损伤有保护作用。机制与增加血总 SOD、降低血MDA的含量，降低多元醇代谢路径关键酶血清AR的含量，保护 髓鞘细胞，改善神经组织脱髓鞘现象，提高神经传导速度，提高周围神经 神经营养因子的含量有关。 关键词：六味地黄丸；2型糖尿病；胰岛素抵抗；周围神经病变；血清 MDA；血总SOD；血清AR；神经营养因子 Research on the therapeutic effect and mechanism of liuwei dihuang pill in rats with type 2 diabetes peripheral neuropathy Abstract: bjective: by observing the impact of Liu Wei Di Huang pills on type 2 diabetes fasting blood glucose, body weight, fasting insulin ,insulin resistance index, total blood SOD, serum MDA, blood AR, sciatic nerve NGF mRNA expression and sciatic nerve lesions, to evaluate the therapeutic effect of type 2 diabetes, to explore its therapeutic mechanism of action, and to provide the theoretical basis in improving DPN of Liuwei dihuang pill Methods : 8-week-old male SD rats were randomly divided into normal controls and modeling ones. Normal controls were offered a standard diet,and a high-fat high-sugar fed were givern to the remaining rats for eight weeks. 8 weeks later injecting STZ in a small dose intraperitoneally to create the type 2 diabetic rat model. 72 hours later after the injection , detecting each rat’s fasting blood glucose (FBG) and fasting insulin(FINS) levels to assess insulin resistance index (IRI). Judging modeling success by IRI 1.20 and FBG 7.0mmol / L. Dividing the rats meeting diabetic standards into type 2 diabetes model group, Tangmoning controls, Liuweidihuang pill high, medium and low dose controls. All treatment rats were orally given drug for 8 weeks according to their body surface area,and weighed weight in 1 week, 4 weeks, 8 weeks after treatment .At 8th week after the treatment beginning measuring fasting blood glucose levels through venous blood, nerve conduction velocity(NCV), fasting insulin by radioimmunoassays, aldose reductase(AR) by ELISA, total superoxide dismutase( SOD) and serum malondialdehyde（MDA）,after Pic