六味地黄方含药血清及其入血成分对大鼠前脂肪细胞增殖、分化的影响-中医内科学专业论文.docxVIP

EFFECTS EFFECTS 0F LIUWEIDIHUANG DRUG—CONTAINING SERUM AND ITS 0RIGlNAL CONSTITUENTS MIGRATING T0 BLOOD ON THE PROLIFERATl0N AND DIFFERENTIATl0N OF RAT PREADIPOCYTE ABSTRACT 【Objective]To confer the effects of Liuweidihuang drug---containing serum and its original constituents migrating to blood on the CAMP cGMP proliferation differentiation and the gene expression of peroxisome proliferator--activated receptor·y2(PPARv2)of rat preadipocyte cells [MethodsI Firstly，the three original constituents migrating to blood were identified and determined by RP--HPLC．Secondly，Liuwei dihuang drug--containing serum and its three original constituents migrating to blood were added to the culture media of rat preadipocytes．The proliferation of cells，the expression of GPDH and lipid droplet accumulation in cytoplasm were observed and recorded．Thirdly，The changes of cAMP and cGMP contents were determined by using competitive protein binding assay, The last，[he effects of Liuweidihuang drug---containing serum on the PPAK2 gene and protein expression were assayed by semiquantitative RT．PCR 【Results]Loganin．paeoniflorin and paeonol were identified as the original constituents Liuweidihuang decoction migrating to blood．in the situation of yin—asthenia，the contents were more than normal．Compared with pills and decoction，they were the highest in micro—powder．r,iuwei dihuang drug---containing serum and its three original constituents migrating to blood blood stimulated rat preadipocyte proliferation、inhibited GPDH up-regulation during differentiation、 inhibited lipid accumulation in cell differentiation、decreased the amount of cAMP and restrained the expression of PPA艮2 mRNA in rat preadipocyte cells，loganin was one of the main constituents which decreased the amount of cAMP micro—powder not only kept the effects of traditional decoction pieces，but also was better than pills and decoction． 【Conclusion]Firstly，the effects of Liuweidihuang prescription on the