六味地黄汤对大鼠单侧输尿管梗阻后肾小管间质TIMP1和ColⅣ的影响-中西结合临床(内科)专业论文.docxVIP

中文摘要实验目的：通过观察六味地黄汤对大鼠单侧输尿管funi 中文摘要 实验目的：通过观察六味地黄汤对大鼠单侧输尿管funi latera卜 一Lifeteral obst riletion，uuo)N，肾小管间质金属基质蛋白酶组织 抑皋4齐4—1(Ti$SUe inhibitor of metalIoproteinas，TIMP一1)和IV 型胶原(cllagen IV，ColIV)表达的影响，探讨六味地黄汤在l净小 管间质纤维化中的作用机制。 实验方法：利用大鼠单侧输尿管梗阻模型，观察六味地黄汤对UUO 大鼠3天、7天、14天共三个时间点的肾小管间质组织形态学及TIMP一1 和col IV表达的影响，并与依那普利组、模型组、假手术组进行比较 实验结果： 1、UUO模型复制成功； 2、六味地黄汤减轻了UUO大鼠肾小管间质的病变； 3、六味她黄汤下调UUO大鼠肾小管间质TIMP一1的达； 4、六味地黄汤下调UUO大鼠肾小管间质fiOCol IV表达。 实验结论：六味地黄汤可下调TIMP一1和colIV的表达，减缓UUO大鼠 肾小管间质病变，可作为梗阻性’肾病辅助用药。 关键词：单侧输尿管梗阳，舟未地黄汤，细胞外基质，TIMP—i，CoIIV The The Experimental Study on the Effects of ljuwejdihuang Decoction on the UUO Rats and CoIIV、TIMP-1 Expression Writer：Yu Yamin Adviser：He Zeyun (Hunan college ofTraditional Chinese Medicine，ChangSha，410007) ABSTRACT objective：To observe the effects of LIUweidihuang Decoction to Unila- -teral ureter obstruction(UUO)rats by tissue inhibitor of metalloproteinase一1 (TIMP-1)and collagen type IV(collV)，investigated the mechanism of renal Tubuloinferstitial fibrosis Methods：With Sprapue-Dewley rat models UUO operation，this search oberserved the effects of LIUweidihuang Decoction on pathologic signs of tubulointerstitial and the expression of the TIMP一1 and collV in the renal tubulointerstitial．Let compare LIUweidihuang Decoction group(Z)with sham surgery group(J)，model group(M)and Enalapril group(Y) Results：1．UUO was copyed successfully 2．LIUweidihuang Decoction alleviated tubulointerstitiat pathology of UU0 rats 3。LlUweidihua玎g Decoction degraded the expression of TIMP—l in TllbulointerstiIial 4，LIUweidihuang Decoction degraded the expression of collV in Tubu一 一lointerstitial Conclusion：LIUweidihuang Conclusion：LIUweidihuang Decoction degraded the expression of TIMP．1 and coilV in]mbuloinCerstitial and alleviated tubulointersIitial pathology ofUUO rats．It can be assistant medicine on obstr，uction renal disease． Key words：UUO；Liuweidihuang decoction；extracelluar matrix(ECM)； collagen IV(CollV)；tissue inhibitor metalJoproIeinase·I(TIMP-1) 缩略词袁缩略词 缩略词