光动力疗法对小鼠B细胞淋巴瘤的体外和体内抗肿瘤作用-肿瘤防治研究.PDF

2014 41 8  Cancer Res Prev Treat,2014,Vol.41,No.8 861 肿瘤防治研究 年第 卷第 期 · · doi:10.3971/j.issn.1000-8578.2014.08.001 ·基础研究· 光动力疗法对小鼠B细胞淋巴瘤的体外和体内 抗肿瘤作用 文兰英 Anti-tumor Effects of Photodynamic Therapy on Mouse B Cell Lymphoma in vitro and in vivo WEN Lanying Department of Obstetrics and Gynaecology, Yanbian University Hospital,Yanji 133000,China Abstract: Objective To investigate the anti-tumor effects of photodynamic therapy(PDT) using Photodithazine, a chlorin e6 derivatives, as photosensitizer(PS) on mouse B cell lymphoma both in vitro and in vivo. Methods After treated with various concentrations (0.125-1.0 μg/ml) of PS, A20 cells were illuminated with 6.25 J/cm2 of laser [wavelength (662 ± 3) nm]. WST-1 assay was performed to detect the cell proliferation, electron microscopy was used to observe morphological changes, and flow cytometry(FACS) was applied to detect the apoptosis rate and the expression of B cell specific antigen-CD45R. We established lymphoma animal model by subcutaneously injecting A20 cells into Balb/c mice. Tumor growth was recorded after PDT intervening in vivo. The expressions of P53, P21 and Bax in tumor tissue were detected by Western blot. Results After PDT for 24 h, the growth of A20 cells was inhibited in the group with 0.50 μg/ml of PDT(P0.05), moreover, there was significant inhibition in the group with 0.25 μg/ml of PDT after 48 h (P0.01). Apoptotic and necrotic cells were detected under the microscope. We successfully established lymphoma animal model and tumor growth was inhibited by PDT in vivo(P0.05). P53, P21 and Bax were highly expressed in tumor tissues which accepted PDT in vivo. Conclusion Photodithazine/PDT has anti-tumor effects on A20 lymphoma both in vitro and in vivo. Key words: Photodynamic therapy(PDT