微生物-生物促生剂协同修复河道底泥-中国环境科学.PDF

中国环境科学 2019,39(1) ：351~357 China Environmental Science 微生物-生物促生剂协同修复河道底泥 ——促生剂投量对修复效果的影响 * 孙井梅 ,刘晓朵,汤茵琪,李 檬,邹亚男 (天津大学环境科学与工程学院,天津 300350) 摘要：采用 5 个 110L 的模拟河道反应器,在投加菌剂的基础上(底泥稳定后向底泥和水中分别注射浓度为 0.09%和 0.03%的菌剂),生物促生剂投量分别设计 为 0.08,0.09,0.10 和 0.11g/L,单独投加菌剂组作为空白实验.通过为期四十余天的实验研究微生物与促生剂协同作用时的修复效果.结果表明,促生剂投量为 0.10g/L 时,上覆水 COD 去除率 69%高于其余各组;各协同修复组上覆水中NO3--N 基本无积累,且促生剂投量为 0.11g/L 时NO3--N 去除率最高,可达 96%;40d 内各组底泥 TOC,TN 含量变化不显著,延长反应时间至 84d,各协同修复组底泥 TOC,TN 去除率均有明显提升.各协同修复组脲酶活性均波动下降,蛋白酶活 性在 15d 左右时达到峰值,增幅可达 106%~237%.通过高通量测序技术发现,43d 时 Desulfuromonas, Pseudomonas, Treponema_2, Blvii28_wastewater- sludge_group 等主要功能菌群的相对丰度与空白实验组相比均增加,且促生剂投量为 0.10g/L 可使微生物群落向更适宜降解去除氮素及有机质的方向演替, 有效改善底泥环境.协同修复对底泥,上覆水水质及微生物群落有良好改善效果,且促生剂投量为 0.1g/L 时整体修复情况更为理想. 关键词：底泥修复；高通量测序；酶活性；微生物群落结构 中图分类号：X522 文献标识码：A 文章编号：1000-6923(2019)01-0351-07 Microorganism and biostimulant collaboratively remediate river sediment—Influence of biostimulant quantity on repair * performance. SUN Jing-mei , LIU Xiao-duo, TANG Yin-qi, LI Meng, ZOU Ya-nan (School of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China). China Environmental Science, 2019,39(1)：351~357 Abstract ：By using five 110L simulated river reactors, the biostimulant dosage was designed as 0.08, 0.09, 0.10 and 0.11g/L, respectively, with adding aerobic denitrifiers (sediment and water were injected at a concentration of 0.09% and 0.03% of the agent), and the aerobic denitrifiers supplement group was used as a blank experiment, to study the repair effect in the collaborative effect of microorganism and biostimulant through more than 40days’ experiment. The results showed that when the dosage of biostimulant was 0.10g/L, and the removal rate of COD in overlying water was 69%, wh