森林脑炎患者血清抗体水平的监测研究-中(蒙)西医结合临床专业论文.docxVIP

摘 摘 要 目的：观察森林脑炎患者特异性抗体出现的时间规律，并对抗体滴度与病情的严 重程度之I．白J的相关性进行研究，从而探讨森林脑炎患者体内的免疫应答情况。方法： 选取2000年一2010年门诊及临床的蜱叮咬者。采集蜱叮咬者静脉血5．0ml，离心后抽 I 取血清装入血清管中加封编号，存放于低温(4℃)冰箱内待检。应用问接免疫荧光法 ， (IFAT)对蜱叮咬患者的血清进行特异性抗体IgM及IgG的检测。然后对IgM及lgG 的出现时间及抗体滴度进行记录。根掘不同年份、不同性别、不同年龄组、不同病情 对抗体出现的时问进行整理，并对不同病情患者的抗体滴度进行整理、分析。对．f：述 结果采用Excel 2003和SPSSl7．0软件进行统计分析。结粜：住抗体Ⅲ现的时11lJ规律 方面，研究发现森林脑炎特异性IgM抗体出现的时I’甘J约d川‘咬后6一10天，Ig(；抗体出 现的时I’日J约在ll—15天。中度和重度森林脑炎患者IgM及IgG抗体：{j现时1．HJ较轻_f：叟：患 者晚。3卜50岁患者抗体出现的时1．日J早于其它年龄纠【患抨。在抗体滴度与病til}41I天性 的研究方面．研究发现重度森林脑炎患者lgM抗体、I，均滴度高r轻度忠抒。结沦：J逦 用免疫荧光法检测特异性lgM抗体造‘一期珍断森林腑炎柄J糸的较为理恕的办法．If．·叮 根掘森林脑炎特异性IgM抗体的滴度判断森林脑炎n，J俩t。l， 关键词：森林腑炎：血清：抗体：监测研究 咱 ....._ The The Monitoring of Serum Antibody LeVels of Tick—bome Encephalitis Patients Abstract ObjectiVe：In order t0 ob辩rve the陀gularity of the time appear in patientS oflhe tick．bome encephaIitis． 绷d re辩arch the co九．elation bet、veen the antibody drops degrees绷d the辩Verity Of the川ness．Thus to ，．{≯ inVestigate the immune瞪ponse of the tick·bome encephalitiS patientS．Methods：Select year 2000·20 l O outpatient锄d cIinic引tickS bites people．Ex妇ction the Venous b100d 5．Oml of ticks bites，centri如gal aner extraction of senIm load semm tube seal Numbers．stored in low temperanlre(4℃)re衔gerator．Indirect immunofluorescence锄tibody technique《I FAT) w弱uSed to detect tIle spec湎c IgM彻d lgG antibodies in the serum of patientS with tick bites．111en record ttle IgM and IgG time of occurrence and antibody drops degrees．Son out the antibodies appear time according t0 different yearS卸d di行brent gender．difrerent age groups and di脓rent condition．Meanwhile．Son out and analysis the patient’s antibodj drops base on the di行-erent condition．ExceI 2003 and SPSS l 7．0 were used in the statisticaI 锄alysis of results．Results：ln the time of appearance rules in antib‘)dj．the studj t-ound t11at the specinc IgM antibody of encephalitis out time about the incidencc aner 6-l 0 daj s．Ig【j antibodies appear in time about ll-l 5 da，s．And in patients with Se、cre t’0rcst cnccphaIⅢs IgM a