利用牙髓干细胞进行牙齿再生的初步研究-动物学专业论文.docxVIP

y y 61 91 88 Acknowledgement The thesis has drowned upon the advice，encouragement and support of a good number ofteachers and schocllmates． First and foremost，my sincere thanks go to Professor Yanding Zhang,my supervisor,who has been instructing me all kinds ofknowledge and technique， and providing me with advice，help and support in laboratory and life during my 3 years postgraduate life．There would not be my thesis without Professor Yanding Zhang．My gratitude also goes to Professor Jianmin Gao for his understanding，caring and encouragement．It is him that gave me the chance to get into the advanced biology． My heartfelt thanks go to Dr Jiang Chen and Dr Xi Xie for their kindly providing me with patient’S teeth for my experience．My thanks also go to Ms Fei Wang and Mr Yide Huang for their kindly help．I must express my gratitude to our schoolmates—Chao Liu，Peng Jiao，especially to Yinqing Dai who has done SO much for my thesis： Finally,1 would like to express my thanks to the all the people who have helped me during my postgraduate study． 辫青、导师冈南 纫垒文公布 内容提要牙齿再生的最终目的就是利用人体自身的组织在牙齿缺失的部位再 内容提要 牙齿再生的最终目的就是利用人体自身的组织在牙齿缺失的部位再 生出牙齿来。牙齿褥生的研究是一个长期而且艰难的过程，本研究只是对 利用牙髓干细胞进行牙齿再生做了初步的探索。主要研究工作包括；建立 一种能利用分散的细胞进行再生牙齿的技术；了解人类牙齿形态发育；确 定模式动物；制备ALU探针；分离鉴定牙髓干细胞；以及牙髓于细胞与 人牙上皮的重组和人牙上皮与鼠问充质的重组实验。实验结果表明，我们 所建立再生牙齿的方法是可行的，能在小鼠的肾囊膜上长出发育良好的再 生牙齿。我们还发现人类牙齿的发育与小鼠的牙齿发育相类似。牙原基在 裸鼠体内能形成完整的牙齿结构，说明裸鼠是一个很好的模式动物，用于 进行人类牙齿的再生研究。分离的牙髓干细胞具有很高的增殖能力，并能 分化形成钙结节。在重组实验中，我们发现重组物都不能形成牙齿，只是 发育成一些类骨质的结构。但是由于时间的关系，确切的结果还需要进一 步的验证。 关键词：牙齿发育，牙齿再生，重组，移植 AbstractThe Abstract The long-term goal of tooth regeneration is to create human teeth，and eventually,to enable patients to regenerate their own teeth following tooth loss using their own tissues．It was a long—term project，so we only did the early study and elementary exploration about tooth regeneration with dental pulp stem cells(DPSCs)．In this study,we established a feasible technique for tooth regeneration in mice by using culture cells．We demonstrated that human too