卵巢癌中NOS和VEGF的表达及其与肿瘤血管形成关系的研究-妇产科学专业论文.docxVIP

Y-618202中文摘要 Y-618202 中文摘要 卵巢癌中NOS和VEGF的表达及其 与肿瘤血管形成关系的研究 摘 要 目的 探讨eNOS、iNOS和VEGF在卵巢癌中的表达及其与肿瘤血管形 成的关系。 方法 免疫组化方法检测61例卵巢癌患者手术切除的石蜡包埋标本中内 皮型一氧化氮合酶(eNOS)、诱导型一氧化氮合酶(iNOS)、血管内皮生长因子 (VEGF)的表达。CD。显示血管内皮细胞，根据CD。。阳性的血管内皮细胞计数 来测定肿瘤微血管密度(MVD)。 结果①eNOS、iNOS阳性和VEGF阳性在卵巢癌中表达率分别57．4％， 70．5％，59．0％，②iN0s和VEGF的表达在卵巢癌淋巴结转移组中明显高于 无淋巴结转移组(Po．05)，iNOS和VEGF的表达随卵巢癌组织分化程度降低 而增高(PO．05)，而与病理类型、临床分期无关(PO．05)。③iNOS表达阳性 的卵巢癌组织MVD：53．6士11．3／每高倍视野，显著高于阴性组28．74-6．7／每 高倍视野，(P0．001)。VEGF表达阳性的卵巢癌组织MVD：46．9±10．3／每 高倍视野，而阴性组织则为32．5±6．z／每高倍视野，差异有高度统计意义(P 0．001)。eNOS表达阳性的卵巢癌组织MVD与阴性组织无显著性差异(P 0．05)。④iNOS的表达与VEGF的表达有相关性(PO．01)。 结论iNOS、VEGF可促进卵巢癌肿瘤血管形成，iNOs在VEGF诱导卵 巢癌肿瘤血管形成中起重要作用。 关键词 卵巢肿瘤；癌；一氧化氮合酶；血管内皮生长因子；肿瘤血管形 成 研究生：吴 洁(妇产科学) 导师：纪新强 夏文捅妥——√——————————————～————————————————————————————————————————————————————————————————————————一———————一 夏文捅妥 ——√——————————————～————————————————————————————————————————————————————————————————————————一———————一 THE STUDY 0F NITRIC 0XIDE SYNTHASE AND VASCULAR ENDOTHELIAL GROWTH FACTOR EXPRESSION lN OVARIAN CANCER AND THEIR RELATION TO ANGIOGENESIS ABSTRACT objective To mvestigate endothelial aitrlc oxide synthase(eNOS)，inducible nitric ox— ide synthase(iNOS)and vascular endothelial growth factor(VEGF)protein expression in o— varian eancer and their role in angiogenesis． Methods Tissue sections from 61 ovarian cancer patients were examined immunohis— tochemieally for protein expression of eNOS、iNOS、VEGF．Microvessal density(MV0) was counted by endothelial cells immunostained by anti—CD¨antibody．Results ①The positive rate of eNOS．iNOS and VEGF was 57．4％。70．5％and 59．O％in ovarian cane— er．②The expression fates of iNOS and VEGF in patients with lymph node metastasis in ovarian cancer were significantly higher than those without lymph node metastasis。(P 0．05)．There was negative correlation between the expressitm rate of iNOS and VEGF and histologie classificationt(P0．05)．@MVD was 53．6±11．3／HP and 46．9±10．3／HP in ovarian cancer postive for iNOS and VEGF t,vhik it was Z8．7士6．7，HP and 32