快眼动睡眠剥夺对抑郁模型大鼠脑内5-HT1A受体mRNA的影响-精神病与精神卫生学专业论文.docxVIP

汕头大学医学院硕士学位论文 汕头大学医学院硕士学位论文 PAGE PAGE VI mRNA 转录较 REMSD 时有降低趋势，但差异无显著性(P＝0.608)；大平 台对照组经 72 小时水环境处理对海马 5-HT1A 受体 mRNA 转录水平没有影 响（P＝0.786）。 3. 21 天慢性应激后，额叶 5-HT1A mRNA 转录水平与正常对照组相比下 降(P＝0.005)，而 72 小时 REMSD 后 5-HT1A 受体 mRNA 表达与抑郁组相 比有增多趋势，但差别无统计学意义(P0.05)，48 小时恢复睡眠后 5-HT1A 受体 mRNA 转录较 REMSD 时有降低趋势，但差异无显著性(P＝0.608)。 4. 21 天慢性应激之后，中脑部位 5-HT1A 受体 mRNA 转录水平与正常对 照组相比显著增加（P＝0.026），72 小时 REMSD 后转录水平与抑郁组相比 显著下调（P＝0.028），48 小时恢复性睡眠之后比 REMSD 时有增加趋势， 但差别无统计学意义（P＝0.238）。 5. 21 天慢性应激、72 小时 REMSD 及 48 小时恢复性睡眠对各组大鼠 下丘脑部位的 5-HT1A 受体 mRNA 表达含量改变无统计学意义（P＝0.715）。 结论： REMSD 具有快速抗抑郁效应，其机制可能与 REMSD 导致的 中脑和海马区 5-HT1A 受体 mRNA 的表达改变有关。 关键词 快眼动睡眠剥夺；抑郁症；慢性轻度不可预见性应激；5-HT1A 受体 Effects of REM Sleep Deprivation on the Expression of 5-HT1A receptor mRNA in the Brain of Rat with chronic stresses Postgraduate Zhang Yinnan Supervisor Prof. Xu Chongtao Abstract Background and Objective: One of the most impressive outcomes of sleep deprivation is its dramatic antidepressant effect in patients with primary depression. One night sleep deprivation exert more rapid antidepressant efficacy. Puzzlingly, such effect is transient and depression is usually reinstated after the first episode of recovery sleep, or even after short naps .To date, the mechanism of the acute antidepressant effect of sleep deprivation has not been clarified. Sleep deprivation for one night induces mood improvement in depressed patients, an action that probably involves the serotonergic (5-HT) system. 5-HT1A receptors located on the cell body and dendrites of 5-HT neurons (autoreceptors) play a key role in this regard. To investigate the mechanisms of this antidepressant effects during sleep deprivation and the possible involvement of 5-HT1A receptor, we studied the effects of an 72-hour REM (rapid eye movement) sleep deprivation, by flowerpot technique, and recovery sleep on the expression of 5-HT1A receptor mRNA in corresponding brain in chronic mild unpredictable stress (CMUS) animal model. Materials and Methods: Sprague-Dawley r