鱼腥草总黄酮的提取纯化及黄酮类型的初步鉴定.pdf

※工艺技术 食品科学 2007, Vol. 28, No. 09 287 郭艳华，许江扬 (江汉大学化学与环境工程学院，湖北 武汉 430056) ：研究了乙醇提取鱼腥草黄酮的最佳工艺，并测定其含量。结果表明：鱼腥草黄酮得率最高的提取工艺 为：用乙醇浓度为60%的溶液浸提，鱼腥草干粉质量(g)与乙醇溶液体积(ml)比例为1:50，提取温度为70℃，时间 为70min，使用聚酰胺对提取物中的黄酮类化合物进行纯化处理，得率为0.586%。对鱼腥草黄酮进行了理化性质、 薄层色谱和紫外- 可见光谱分析，初步确定鱼腥草黄酮为黄酮醇类化合物。 ：鱼腥草；黄酮；黄酮醇；紫外 - 可见光谱 Extraction and Purification of Houttuyniae cordata Flavonoids and Identification of Flavonoids Type GUO Yan-hua，XU Jiang-yang (School of Chemistry and Enviornmental Engineering, Jianghan University, Wuhan 430056, China) Abstract ：In this research, extraction of flavonoids-natural antioxidant in Houttuyniae cordata by alcohol was studied. Meanwhile, contents of flavonoids were assayed. Results showed that the 60% density alcohol as extraction solution, the Houttuyniae cordata powder quantity with alcohol solution volume 1:50, temperature 70 ℃ and optimum conditions are: Houttuyniae cordata flavonoids were purified by polyamide, and the yield of flavonoids reached the time 70 min. The Houttuyniae cordata flavonoids was identified as flavonol by physical and chemical properties analysis, TLC and 0.586%. UV-VIS. Houttuyniae cordata；flavonoids；flavonol；UV-VIS Key words： 中图分类号：Q946.885.9 文献标识码：A 文章编号：1002-6630(2007)09-0287-05 鱼腥草为三白草科蕺菜属植物蕺菜的全草，始载于 1.1 材料与仪器 《名医别录》，是国家卫生部正式确定为“药食两用” 1.1.1 材料和试剂 的品种之一，其性味辛、寒, 具有清热解毒、消肿排 鱼腥草 武汉隆泰医药公司。 脓、利尿涌淋的功效。鱼腥草主要含有丰富的黄酮类 聚酰胺、硅胶 G 、无水乙醇、甲醇、丙酮、乙 化