酿酒酵母新的DNA复制起始蛋白的筛选.pdfVIP

缩写词 缩写词 全名 ACS ARSconsensus sequence Amp amplicilin ARS autonomouslyreplicatingsequence ATP adenosine 5’-triphosphate BSA bovineserumalbumin CDC celldivision cycle CDK kinase cyclindependent CllIP chromatin immunoprecipitation DDK kinase dbf4-dependent DMS0 sulfoxide dimethyl EB ethidium bromide EDTA acid ethylenediaminetetraacetic EMS methanesulfonate ethyl IDRoridr initiationofDNA replicaiton IP immunoprecipitation LB Luria—broth medium MCM mini—chromosomemaintenance MCS site multiple-cloning OD opticaldensity ORC origin recognitioncomplex PCR chainreaction polymerase PEG Polyethylene glycerol P0st．RC post-replicative complex Pre．IC pre-initiation complex Pre．RC pre—replciative complex RNaseA ribonucleaseA rounds rpm minute pe