多种抗原对树突状细胞促成熟作用的研究.docxVIP

多种抗原对树突状细胞促成熟作用的研究 田发青1季德兰2李举亨1唐玫琴1李惠卿3黄映彩3成小慧1 （1深圳市龙岗区人民医院血液内科 广东 深圳518172） （2深圳市龙岗区人民医院中央悦城社康 广东 深圳518172） （3深圳市龙岗区人民医院血细胞实验室 广东 深圳518172） 【摘要】目的：研究多种抗原促进树突状细胞（dendritic cells, DCs）成熟 作用。方法：外周血单核细胞培养为DC,分别负载卡介苗、肺炎球菌疫苗、麻 疹疫苗、K562及RPMI8266冻溶抗原后与T淋巴细胞共培养，以单独加入TNF-a 为对照组，再加入相同体积培养基作为空白对照，观察DC形态，流式细胞仪分 析DC表型，CCK8法测定混合淋巴细胞反应（MLR）。结果：各组均培养出具有 典型形态的DC,其中疫苗各组细胞CD83表达较肿瘤细胞各组明显升高，MLR 作用强，而肿瘤组抗原乂较对照组和空白对照组为高，同时各实验组CDla表达 较对照组和空白对照组明显降低，Plt;0.05,但各实验组CDla表达未见明显差 异，Pgt;0.05o结论：卡介苗、肺炎球菌疫苗及麻疹疫苗等微生物抗原促进DC 成熟能力较K562及RPMI8266等肿瘤抗原能力强。 【关键词】抗原；树突状细胞；CD83；混合淋巴细胞反应 【中图分类号】R331.1+44 【文献标识码】A 【文章编号】2095-1752 （2016） 08-0385-02 Multiple kinds of antigen induce dendritic cells to maturation ITian Faqing, 2Ji Delan 1LI Juheng, ITang Meiqin, 3LI Huiqing, 3Huang Yingcai,lCheng Xiaohui IDepartment of Hematology, Longgang District Peoplersquo;s Hospital of Shenzhen; 2Blood Cell Laboratory, Longgang District Peoplersquo;s Hospital of Shenzhen, Guangdong, 518172, China 【Abstract] Objective To investigate the capability of multiple kinds of antigens(Ags) to promote dendritic cells (DCs) mature.Methods DCs were induced from peripheral blood mononuclear cells loaded different Ags such as BCG, pneumococcal vaccine，measles vaccine，K562 and RPMI8266-freeze-thaw Ags, then to be co-cultured with T lymphocytes. TNF-a only was added as control group, and TNF-a and the same volume of medium as Ag were added as a blank control. DC morphology was observed by in verted microscope, DC phe no type was an alyzed by flow cytometry, and mixed lymphocyte reaction (MLR) was determined by CCK8 kits. Results Cells in each group were induced to have the typical morphology of DC, which CD83 expression was significantly higher in vaccine groups than in tumor cell groups, and was higher in tumor cell groups than in control group and blank control group, while CDla expression was sign讦icantly lower in each experimental group than in control group and blank control group, Plt;0.05, but CDla expression in each experimental g