NF-КB在兔蛛网膜下腔出血模型基底动脉中的表达及其与血管平滑肌增殖的关系.docVIP

NF-КB在兔蛛网膜下腔出血模型基底动脉中的表达及其与血管平滑肌增殖的关系 张建忠，周 政，刘 俊，杨 辉，黄 巍，张 旋，冯宝海 （400037 重庆，第三军医大学新桥医院神经外科） [摘要] 目的 观察兔蛛网膜下腔出血(subarachnoid hemorrhage，SAH)模型基底动脉中核转录因子（nuclear factor-КB， NF-КB)和增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）的时间动态表达情况，并分析NF-КB与SAH诱导的基底动脉壁增殖的关系。方法 40只雄性新西兰大白兔随机分为假手术组和SAH组（n=5）。SAH组采用枕大池二次注血法建立脑血管痉挛动物模型，假手术组注入等量生理盐水，各组于二次注血术后第1、4、7、14 d观察基底动脉组织病理学改变，并应用免疫组化、Western blot法分析各组基底动脉中NF-КB、PCNA 阳性细胞比值和蛋白水平的表达差异。结果 与假手术组相比，SAH组基底动脉血管壁平滑肌随观察时间逐渐增厚，管腔狭窄，在第7天达高峰(P＜0.05)，在第14天时恢复至正常水平；NF-КB和PCNA蛋白表达随时间逐渐增强，在第7天达高峰(P＜0.05)，在第14天时表达下降。NF-КB的表达与基底动脉平滑肌厚度变化及PCNA表达变化呈显著正相关(r =0.80和r =0.75， P＜0.05。）结论 NF-КB的激活可能参与了SAH后脑血管平滑肌细胞增殖，NF-КB可能通过转录多种促血管增殖因子参与SAH诱导的血管壁增殖过程。 [关键词]蛛网膜下腔出血；核转录因子；平滑肌细胞；增殖 The relationship between expression of NF-КB and vascular proliferation in the rabbit model of subarachnoid hemorrhage Zhang Jian zhong，Zhou Zheng，Liu Jun，Yang Hui，Huang Wei，Zhang Xuang，Feng Bao hai (Department of neurosurgery，Xinqiao hospital，Third Military Medical University，Chongqing 400037，China) [ABSTRACT] Objective Aim to investigate rabbit model of subarachnoid hemorrhage(SAH)in the basilar artery of nuclear transcription factor (nuclear factor-КB， NF-КB) and PCNA (proliferating cell nuclear antigen， PCNA) expression dynamics over time and analyze the NF-КB and basilar artery wall proliferation. Methods Forty New Zealand male rabbits were randomly divided into Sham group and SAH groups（n=5）。Using double injections of fresh autologous arterial blood into the cisterna magna to establish animal models of cerebral vasospasm． After injection 1st，4th，7th and 14th day，the expression of NF-КB and PCNA of basilar artery in rabbits were determined by immunohistochemistry and Western blot；morphological changes of rabbit basilar artery was also observered. Results Compared with Sham group， the results showed SAH group with the observation period blood gradually smooth muscle wall thickening， stenosis， and peaked on the 7th day(P＜0.05). The protein expression of NF-КB of