PPAR-γ激动剂促大鼠急性心肌梗死后血管新生与eNOS表达调控.docVIP

编号： 刊用形式：论 著 文章编号： PPAR-γ激动剂促大鼠急性心肌梗死后血管新生与eNOS表达调控 何 泉,雷 寒,柳 青,覃 数,马康华,王 惠 （重庆医科大学附属第一医院心血管内科，重庆 400016） 摘 要：目的 研究PPAR-γ激动剂罗格列酮对大鼠急性心肌梗死（acute myocardial infarction,AMI）后血管新生及内皮型一氧化氮合酶（endothelial nitric oxide synthase，eNOS）表达调控的影响。方法 健康成年SD大鼠60只，用随机数字表法分为假手术组、对照组、罗格列酮组、罗格列酮+L-NAME(NOS抑制剂)组、L-NAME组，每组12只。结扎大鼠冠状动脉左前降支建立急性心肌梗死动物模型。灌胃给予罗格列酮(3 mg·kg-1·d-1 )2周，同时腹腔内注射给予L-NAME(40mg·kg-1·d-1 )2周。检测梗死周围缺血区CD31阳性染色血管数；以Western blot及RT-PCR检测缺血区丝氨酸1177磷酸化内皮型一氧化氮合酶(phosphoralated endothelial nitric oxide synthase at Ser1177,p-eNOS Ser1177)蛋白及eNOS mRNA的表达。结果 ①与对照组比较，罗格列酮组心肌梗死后CD31阳性染色血管数显著增加（P0.05），而NOS抑制剂L-NAME则抑制了罗格列酮的这一作用。②罗格列酮组eNOS mRNA表达与对照组比较差异无统计学意义（P0.05），而p-eNOS Ser1177蛋白水平显著升高(P0.05)。结论 罗格列酮能促进大鼠急性心肌梗死后血管新生，其机制可能与其促进eNOS的磷酸化，从而介导内皮源性一氧化氮（endothelium-derived nitric oxide，EDNO）合成增加有关。 关键词：过氧化物酶体增殖物激活受体-γ；急性心肌梗死；血管新生；内皮型一氧化氮合酶 中图法分类号： 文献标识码：A PPAR-γ agonist improves myocardial angiogenesis by regulating Angiogenic Effect of PPAR-γ agonist and regulation of eNOS expression in acute myocardial infarction rats after acute myocardial infarction in rats HE Quan, LEI Han, LIU Qing, QIN Su, MA Kang -Hua, WANG Hui （(Department of Cardiology, The First Affiliated Hospital , Chongqing Medical University, Chongqing 400016, China）) Abstract：Objective To investigate the effects of rosiglitazone , one of the PPAR-γ agonist, on angiogenesis and eNOS expression after experimental myocardial infarction (MI) in rats. Methods Totally 60 healthy adult SD rats were randomly and equally divided into the 5 groups, sham operatedion group、,the control group、, rosiglitazone group、, rosiglitazone plus L-NAME（ (inhibitor of NOS）) group and L-NAME group;. Left anterior descending coronary was undergone permanent occlusion to establish the MI model;. Rosiglitazone （(3 mg·kg-1·d-1) ）was administered via oral gavage, and L-NAME was given by intraperitoneal injection;, In These were followed 2 weeks after the corresponding trea