尖锐湿疣患者治疗前后血清中VEGF、HIF1α水平的测定.docxVIP

尖锐湿疣患者治疗前后血清中VEGF、HIF-1 Q水平的测定 王鲁梅李俊杰林雪香 (广东省东莞市人民医院5 2 30 1 8 ) 【摘要】目的检测尖锐湿疣(CA )患者治疗前后血清中血管内皮生长因子(V EGF )、缺氧诱导因-T-lalpha;(HIF-lalpha;)的表达水平。方法 采用酶联 免疫吸附法(ELISA )检测4 0例尖锐湿疣患者治疗前、治疗后2周、治疗后 1月血清中V EGF、HIF-lalpha;的表达，并与10例健康人进行比较。结果CA 组治疗前血清中VEGF的表达水平明显高于健康对照组(Plt;0.001),差异具有统 计学意义(Plt; 0. 01)o治疗后2周血清中VEGF比治疗前明显下降(Plt; 0. 01), 治疗后1月下降至正常水平，与健康对照组比较差异无统计学意义。CA组治疗 前血清HIF- lalpha;的表达水平与健康对照组比较，差异无统计学意义(Pgt; 0. 01)；治疗后2月、治疗后1月与治疗前比较，无显著性变化。结论CA皮损组 织产生过量的VEGF可能分泌至血液系统中引起血清中VEGF的高水平表达，而H I F - lalpha;不能引起血清中的高水平表达。 【关键词】尖锐湿疣；血管内皮生长因子；缺氧诱导因子alpha;；血清 Detection of Serum VEGF and HIF?lalpha;in Condyloma Acuminatum Before and Afer TreatmentWANG Lu-mei,LI Jiu-jie,LIN Xue-XiangDepartment of Dermatology,Dongguan Peoplersquo;s Hospital,Guangdong 523000,China [Abstract] Objective To delete the serum expression levels of vascular endothelial growth factor(VEGF) and Hypoxia jnducible factor-lalpha; (HIF-lalpha;) before and after treatment in condyloma acuminatum (CA).Methods The serum expressions of VEGF and HIF-1 alpha;in 40 CA patients andlO normal human were detected by enzyme linked—immunosorbent assay technique (ELISA ).Results The serum level of VEGF were significantly higherthan that in nomal human and significantly reduced after treatment.The serum expression of HIF-lalpha; were not significantly higher than that in nomal humanand not significantly reduced after treatment.Conclusion The over-expression of VEGF in CA tissiu induce high level expression of VEGF in serum. Theover-expression of HIF-lalpha;in CA tissiu does not induce high level expression of HIF-lalpha; in serum. 【Key words] Condylomata acuminata； Vascular endothelial growth factor； Hypoxia jnducible factor-1 alpha;； Serum 【中图分类号1R55【文献标识码】B【文章编号]1007-8231 ( 2011) 09-13 13-03 尖锐湿疣(CA)是由人乳头瘤病毒(HPV)感染引起的生殖器部位皮肤 黏膜良性增生性疾病。目前大量免疫组化实验证实，在CA病理组织中存在多种 促血管生成相关因子的高水平表达，其中最为重要的有血管内皮生长因子(VEGF) [1,2]＞缺氧诱导因子(HIF-lalpha;)[3],在促进血管生成方面发挥重要作用。而 CA患者血清中是否存在这两种因子的高水平表达及其与病情严重程度的相关性 如何，国内外尚无相关文献报道。我们采用酶联免疫吸附法(ELISA)检测40例 CA患者治疗前、治疗