小麦钙调素新亚型TaCaM5的克隆及表达分析-作物学报.pdf

作物学报 ACTA AGRONOMICA SINICA 2010, 36(6): 953−960 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.00953 小麦钙调素新亚型TaCaM5 的克隆及表达分析 刘新颖1 王晓杰1,** 薛 杰1 夏 宁1 邓 麟 1 蔡高磊1 汤春蕾1 魏国荣1 黄丽丽1 康振生 1,2,* 1 , 712100; 2 / , 712100 : RT-PCR , CaM cDNA , CaM , TaCaM5TaCaM5 450 bp , 149 ; , 4 EF-hand CaM , TaCaM5 CaM , 97% ; , , 6 h 24 h TaCaM5 , TaCaM5 , TaCaM5 , Ca2+-CaM : ; ; ; RT-PCR Cloning and Expression Analysis of a Novel Calmodulin Isoform TaCaM5 from Wheat 1 1,** 1 1 1 1 LIU Xin-Ying , WANG Xiao-Jie , XIA Ning , DENG Lin , CAI Gao-Lei , TANG Chun-Lei , WEI 1 1 1,2,* Guo-Rong , HUANG Li-Li , and KANG Zhen-Sheng 1 College of Plant Protection, Northwest AF University, Yangling 712100, China; 2 Shaanxi Provincial Key Laboratory of Molecular Biology for Agriculture / Northwest AF University, Yangling 712100, China Abstract: Calmodulin is a ubiquitous transducer of calcium signals in eukaryotes. It mediates a lot of cellular processes, such as transcription, cytoskeletal organization and motility, and amino acid metabolism. In diploid plant species, several isoforms of calmodulin have been described. A novel CaM gene was isolated from cDNA library of wheat leaves infected by Puc