蛋白质合成加工转运相关亚细胞结构.ppt

* Example: intestinal Globet cell * Example: Fibroblast cell in connective tissue RER Secratory Collagen from fibroblast * ProteinTransport to where they Function ribosome Endoplasmic Reticulum Golgi apparatus membrane bound ribosome free ribosome *ER’s proteins *Golgi’s proteins *secratory proteins *lysosomal proteins *plasma membrane protein (video) other proteins: mitochondrial protein, nuclear protein etc. Protein synthesis Protein Processing, sorting * Membrane protein Soluble protein Proteins in organells lumen (ER, Golgi, lysosome) Secretory protein Membrane ER Golgi apparatus * Protein synthesis and processing ribosome ER Golgi Apparatus Cellular organelles work for protein * the translocation reaction involves: the identification and targeting of proteins to the ER, the association of proteins with the ER translocation machinery, including a pore through which proteins enter the ER, the energy-dependent import of proteins into the ER lumen or membrane, protein folding and maturation in the ER secrat * Free ribosome ER membrane-bound ribosome Nascent protein enter into the ER and then go Golgi until terminal destination Destination of Protein Nascent protein enter into the cytosol and then transport to their destination * 1. Proteins that are formed from free ribosomes are released into the cytosol and used within the cell. 2. Proteins that are formed by bound ribosomes usually transported to their destinations through the secretory pathway and used within the plasma membrane, lysosome or are expelled from the cell via exocytosis. Mitochondrion Nucleus peroxisome ER Golgi Apparatus Plasma membrane Lysosme Extracellular matrix Secratory protein * Essential concepts 1. Cells make several functional RNA, including mRNAs, which carry the instructions for making proteins; rRNAs, which are the crucial components of ribosomes; and tRNAs, which act as adaptor molecules in protein synthesis. 2.Translation of the nucleotide sequence of an mRNA int