I标记血管靶向分子在肿瘤显像中的应用-肿瘤防治研究.PDFVIP

8·60 · 肿瘤防治研究 2007 年第 34 卷第 11 期 ·临床诊断 · 13 1 I 标记血管靶向分子在肿瘤显像中的应用 1 2 2 2 1 徐清华 ,粟 　波 ,赵印敏 ,唐 　亮 ,周彩存 Evaluation of Tumor Vascular Targeting Agents Labelled with 13 1 I in Imaging of Tumor XU QingHua1 ,SU Bo2 ,ZHAO YinMin2 , TAN G Liang2 ,ZHOU CaiCun 1 1. D ep a rt ment of Oncology , S hang hai P ul m ona ry H osp i t al , S hang hai 200433 , Chi na , 2 . I ns ti t ute of P ul m ona ry ca rci nom a an d i m m unology Corresp on d i ng A ut hor : Z hou Caicun , Em ai l :caicunz hou @y ahoo. com. cn Abstract :Objective 　 To investigat e t he biological activit y of t umor va scular t ar geting agent s ( R GD 10 、F56 and K237) labelled wit h 13 1 I and t heir sp ecific localization in t umor . Methods 　Tumor va scular t ar geting agent s were labelled wit h Iodogen met ho d and sub sequently sep arat ed wit h SepPak C18 column . Then , t heir immunoreactive f raction and affinit y con st ancc were det ermined aft er HU V ECs t rial s. Their sp ecific localization in nude mice bearing human A549 lung adenocarcinoma were ob served and comp ared by eZ 13 1 13 1 13 1 scop e . Results 　The immunoreactive f raction of IR GD 10 、 IF56 and IK237 was 42 . 7 % 、55 . 1 % and 44 . 5 % , resp ectively . Their affinit y con st ant wit h HU V EC wa s 9 . 4 1 ×107 L/ mol 、3 . 87 ×107 L/ mol and 5 . 98 ×107 L/ mol , resp ectively . 13 1 I R GD 10 locat ed in t umor more den sely t han 13 1 IF56 and 13 1 I 13 1 K237 aft er t hey were inj ect ed into t he t ail vein of nude mice . Conclusion 　 IR GD 10 may be a t ar geting agent in rad