芝麻油中掺入菜籽油的近红外光谱研究.PDF

296 2009, Vol. 30, No. 18 食品科学 ※分析检测 1 1,2, 1 2 冯利辉 ，刘波平 * ，张国文 ，罗 香 (1.南昌大学食品科学与技术国家重点实验室，江西南昌 330047 ； 2.江西省分析测试中心(研究所) ，江西南昌 330029) 采用偏最小二乘法建立芝麻油中菜籽油含量的近红外光谱定量检测模型。配制不同比例的菜籽油和芝麻油 -1 范围内的近红外漫反射光谱，模型采用交互验证和外部检验来考察所建立 混合样品，采集样品在4200～10000cm 模型的可靠性。建模参数为：最佳波长范围为4500～8745cm-1 ；最佳光谱预处理方法为：多元散射校正(MSC)/ 一 阶微分/Norris Derivative(3,5)滤波。所建立定标模型的校正相关系数为0.99839 ；均方估计残差为0.976 。应用建立 的模型对未知样品进行预测，并对预测值和真实值进行比较，在含量为10%～70% 之间范围准确可靠，研究结果 表明，采用近红外光谱技术可以实现芝麻油中菜籽油的快速检测。 近红外光谱；芝麻油；掺假；菜籽油；偏最小二乘 Near Infrared Spectroscopic Determination of Sesame Oil Adulterated with Rapeseed Oil 1 1,2, 1 2 FENG Li-hui ，LIU Bo-ping *，ZHANG Guo-wen ，LUO Xiang (1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China； 2. Jiangxi Province Research of Insititute of Analysis and Test, Nanchang 330029, China) Abstract ：Near-infrared spectroscopy (NIRS) quantitative detection model of sesame oil adulterated with rapeseed oil was established by partial least squares (PLS). Mixed samples of sesame and rapeseed oil with different proportions were scanned using a Thermo-Nicolet Protege-460 FT-IR spectrometer and their near infrared diffuse reflectance spectra were collected in -1 region. The reliability of the model established was verified by cross-validation and external test. The results 4200 –10000 cm showed that the optimum wavelength range was 4500 –8745 cm-1 and the optimum spectra pre-treatment way was multiplicative signal correction (MSC)/first derivative/Norris der