RAPD技术研究6种拟钉螺地遗传变异.docx

华中科技大学同济医学院 硕士毕业论文 genomic DNA．RAPD technique was used to amplified the genomic DNA．Then，the genetic distancegenetic similarity of the tricula from various regions were calculated according to the types of bands of the amplified products the agarose gel electrophoresis，The phylogenetic tree was thereby mapped，and the DNA polymorphic bands detected were analyzed by clustering analysis with spssl 1．0 software． 3．Results ①Observational and comparative of exterior morphological features and ecological environments ofdifferent populmion ofTricula： There are much similarity between Z pingi and Z hsiangi,r hongshanensis and Z xindiangensis in the exterior morphological features and While other species show much diversity． ②DNA extraction： The Az60／A280 value of the DNA solution ranged from qualified．  the habitat environments． 1．60～2．00，the purity is ③RAPD： 1)The difference out of species：The RAPD products of 6 Tricula populmions and Semisulcospira cancellata showed obvious dissimilarity．87 bands，which were homologous to 87 loci of the genome，were amplified with 6 random primers． There ale 5 bands of the 87 bands were the same between the two species．The genetic distance between them was larger than O．66，and the genetic similarity was only O．339 2)The difference between species：Genetic distances among the 6 Tricula populations ranged from O．1301～O．3151．and the average was O．2248．The type ofbands of amplified products between Tpingi and T．hsiangi were much similar．The genetic similarity was 0．8699 and the genetic distance was only 0 1 301．The bands type of Z hongshanens括and Z xindiangensis were much similar．The genetic similarity was O．8558 and the genetic distance was only O．1442，The bands type of these 华中科技大学同济医学院 硕士毕业论文 two snails had some similarity with Hubei—Shiyan isolate in some degree．While Guangxi—Wttming isolate had much diversity to others．The genetic distance among them ranged from O．2234～0．3151． 3)The populations of