大蒜素诱导人胃癌SGC-7901细胞M期阻滞机制研究.docxVIP

·英文论著摘要· Mitotic Arrest Induced by Allicin in SGC-7901 Cell Line and Its Possible Mechanisms Preface Garlic is the bulb of onion genus lily family plant．Epiderniological studies have in —dicated that garlic has some remarkable disease prevention and resistant effects．The effective constituents of garlic mainly contain some organosulfur compounds．Allicin whose chemical name is diallyl trisulfide，is a typical component of them．Allicin has many biologic activities to resist the diseases such as anti—infection，kata-choleste—rol， angio-sclerosis prevention and enhancing the antioxidation and detoxification．Recently， many researches demonstrate that allicin has shown some satisfactory anticancer ef -fects．There are several mechanisms contributed tO its antineoplastic effects．Causing tu -mow cell cycle arrest Was an important way among them．We previously demonstrated that allicin Can induce mitotic arrest in human gastric cell line SGC一7901 and MGC-803．but the accurate mechanism of mitotic arrest causing by allicin has not been established．Vincristine as a M phase specific antitumor drug,Can block tumor cell cycle in M phase，by destroying the stabilization of microtubule dynamics，encouraging the deploymerisition ofmicrotubule and interfering the assembly ofmitotic spindle．The p34。6。2／cyclin B1 complex which Was also called M．phase promoting factor(MPF)is a regulator to the stabilization of microtubule dynamics，by causing phosphorylation of the microtubu le—associated proteins(MAPs)．Is allicin similar to vincristine，Can also destroy the stabilization of microtubule dynamics，and promote the deploymerisition of microtubule?Is the mitotic arrest causing by them regulated by MPF?In this study，we have focused our attention on answering these questions 4 objective We used immunofluoreseence and confocal laser microscope technique tO determine the effect of allicin on inducing mitotic arrest in human gastric cell line SGC一7901，and its possible mecha