牵引成骨中牵引速率对血管生成影响地实验研究.docxVIP

Effect of lengthening rate on angiogenesis during distraction osteogenesis Po stgraduate：Ding wen—be Supervisor：Prof．Tang Xin Maj or subj ect：orthopedics Abstract Distraction osteogenesis generates new bone by utilizing a mechanism for wound healing，which is a new technology．In the bone sture or two sides of broken bone，it utilizes distraction system to distraction by invariably rate．Distraction osteogenesis generates new bone in bone gap to make bone lengthened and widened．It is a kind of endogenic tissue engineering which makes new bone form continuously by distraction to bone cross-section． The new bone moulds in the direction of the distraction．At the same time， the tissues of skin，aponeurosis，nerve，muscle，vessel and cartilage are adaptable rebuilded by the tension of surrounding soft tissue．The slow change Cfln not only avoid the damage to soft tissue，but also ensure the positional stabilization of the new bone．Many studies suggested that the new bone formation was usually accompanied by angiogenesis． New bone formation had c losed relation with angio genesis．So it i s very important that we study the effect of lengthening rate on angiogenesis during distraction osteogenesi S． Objective：This study aims at investigating the effect of four varying rates(0．3，0．7，1．3and 2．7ram／day)of distraction on the vascularization process in rabbit modal of leg-lengthened during distraction osteogenesis． Methods：The experiment used New Zealand White rabbits，The rabbits were randomly divided into four groups，and performed a mid—femur osteotomy model．Each group was lengthened at the rate of 0．3． O．7，1．3 and 2．7mm／day，until the femur was lengthened 2．0 cm than original 1ength．As soon as the appropriate length had been achieved，the regenerating tissues of the distraction gaps were collected．Immunostaining was performed with use of specific antibodies to endothelial cell 3 antigen(CD34)．With using of the Chalkley counting method，staining blood vessel cells were examined sem