红豆杉悬浮细胞放大培养的细胞生长与紫杉醇合成动力学.pdf

6 1 生命科学研究 Vol.6 No. 1 20023 Life Science Research Mar. 2002 X 兰文智, 余龙江, 金文闻, 陈 超 ( , 430074) :研究了在MurashigeskoogÄs(MS)和62 号两种不同的培养基中, 红豆杉细胞悬浮细胞从摇瓶到 10 L 机 械通气搅 式反应器放大培养过程中细胞生长与紫杉醇合成动力学. 结果表明:尽管在不同的培养条件下, 细 胞生长曲线均呈现/ S0型. 紫杉醇在延迟期与指数生长期中基本上没有积累, 而且随着培养规模的增大, 紫杉 醇的含量逐渐降低.进一步对各级放大培养的细胞生长,比生长率与胞内外紫杉醇合成量进行分析, 发现MS 利于细胞生长但不利于紫杉醇合成,而62 号则相反.根据此文的结果,提出了红豆杉细胞培养条件的优化和 大规模细胞培养生产紫杉醇应采取的策略. : 红豆杉细胞; 放大培养;紫杉醇; 细胞生长 + :Q813. 1 1;Q944. 6 : : 1007-7847(2002)01-0055-05 Scale-up Kinetics of Cell Growth and Taxol Biosynthesis in Suspension Cultures of Taxus chinensis Cells L N Wen-zhi, YU Long-jiang, JIN Wen-wen, CHEN Chao ( School of Lfi e Science and Technology , Huazhong University of Science and Technology , Wuhan 430074, Hubei, China) Abstract:The studywas focused on scale-up kinetics of cell growth and taxol biosynthesis in suspension cultures of # Taxus chinensis cells in MurashigeskoogÄs(MS) and 62 media. The results showed that although cellswere culti- vated at different conditions, the growth curves were all / S0 shape. Taxol accumulated only slightly at lag phase and logarithmic phase, and decreased gradually accompanying the scale-up culture. nalyzed cell growth, rate of cell and concentrations of intracellular and extracellular taxol of every scale-up culture, MSwas found to be benefi- # cial to cell growth, bu