MS1516是拟南芥四分体形成和小孢子发育的必需基因.pdf

植 物 分 类 与 资 源 学 报摇 2011,33 (4):383~388 Plant Diversity and Resources摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 DOI:10.3724/ SP.J.1143.2011.11029 MS1516是拟南芥四分体形成和小孢子发育的必需基因* 赵先玲, 常海双, 杨仲南, 张摇 森** (上海师范大学生命与环境科学学院,上海摇 200234) 摘要: 以前报道了雄性育性下降突变体ms1516, 而且图位克隆的方法已将突变基因MS1516定位到拟南芥 基因组第3条染色体上28kb 的区间内。 本文通过进一步的生物信息学分析, 发现该定位区间内有一个与减 数分裂有关的基因AtATM, 而且等位实验结果表明ms1516 和atm鄄3 是等位突变体。 细胞学分析结果表明, ms1516突变体在花药发育过程中产生多个不均等的小孢子, 而且大多数的小孢子不能发育成成熟的花粉。 DAPI染色的结果显示小孢子母细胞减数分裂过程中, 染色体不能正常分离, 对成熟花粉的扫描电镜观察结 果发现突变体多数花粉形态异常。 以上结果说明MS1516基因在小孢子形成和发育过程中具有重要作用。 关键词: 拟南芥; 雄性不育; 四分体; 减数分裂;MS1516基因 中图分类号: Q75摇 摇 摇 摇 摇 摇 摇 文献标识码:A摇 摇 摇 摇 摇 摇 摇 文章编号:2095-0845(2011)04-383-06 MS1516 is Essential for Tetrad Formation and Microspore Development inArabidopsis thaliana ZHAO Xian鄄Ling,CHANG Hai鄄Shuang,YANGZhong鄄Nan,ZHANG Sen** (College of Life and Environment Sciences,ShanghaiNormal University,Shanghai200234,China) Abstract:Anthers in flowers produce via meiosis haploid microspores,which mature to become pollen. However, pollen development at the molecularlevel isstill notvery clear. Forthepurposeof gainingunderstandingof themo鄄 lecular control of pollen development,anArabidopsis partial male鄄sterility mutant ms1516 was reported previously, and the mutant geneMS1516 was mapped to a region of28kb on chromosome3 by a map鄄based cloning approach. Here,we report that further bioinformatics analysis revealed that there was a meiosis related geneAtATM in this re鄄 gion. Allelismtests indicatedthatMS1516 andAtATM belonged to the same locus. Cell biology analysis showed that ms1516 mutant produced multiple uneven microspores during anther development,and most of which aborted at late stages. DAPIstainingindicatedthatchromosomesegregationwasabnormalinms1516mutantmeiocytes. Further scan鄄