脑缺氧缺血后热休克蛋白基因的表达及地塞米松对其的调节.docVIP

???? 脑缺氧缺血后热休克蛋白基因的表达及地塞米松对其的调节 周伟　吴圣楣　陈惠金 　　［摘　要］　目的　探讨热休克蛋白-70(HSP70)基因在缺氧缺血新生大鼠脑内的表达及地塞米松(DEX)对其影响。方法　通过建立新生大鼠缺氧缺血性脑病动物模型，应用快速竞争性RT-PCR技术，分别对缺氧缺血(HI)，DEX预处理后予缺氧缺血(DH)，缺氧结束后即刻予DEX (HD)，DEX加假手术(DS)，正常对照(NS)五组动物的实验侧大脑组织中HSP70 mRNA的表达进行半定量分析。结果　缺氧缺血后新生大鼠脑内HSP70 mRNA的表达明显增强，其表达高峰(3.868±1.421)位于HI后12 h左右，并在12～48 h内维持较高水平(3.186±0.803，2.568±0.709)。DEX预处理可使HSP70 mRNA的表达明显下降或几乎完全被抑制，其12 h，24 h，48 h分别为0.526±0.593，0.492±0.450，0.434±0.428。HI后给予DEX对HSP70 mRNA的表达则无明显影响。其12 h，24 h，48 h分别为3.460±1.309，3.020±0.565，2.242±0.504。结论　DEX预处理可抑制HI对HSP70 mRNA过表达的诱导。推测DEX预处理后使HSP70 mRNA的表达下降，可能与DEX抑制了神经元的过度兴奋有关。　　［关　键　词］　脑；缺氧缺血；地塞米松；HSP70；新生大鼠　　［中图分类号］　R-332　　［文献标识码］　A　　［文章编号］　1008-8830(2000)02-0086-05 Regulatory Effects of Dexamethasone on the Expressionof HSP 70 mRNA in Neonatal Rats Following Cerebral Hypoxia-ischemia ZHOU Wei　WU Sheng-Mei　CHEN Hui-Jin（Xinhua Hospital, Shanghai Second Medical University, Shanghai Institute for Pediatric Research, Shanghai 200092） 　　［Abstract］　Objective　To investigate the expression of HSP70 gene in the brain of neonatal rats following cerebral hypoxia-ischemia (HI) and the effect of dexamethasone (DEX) on the expression of HSP70 mRNA. Methods　Rapid competitive reverse transcriptase-PCR was used to semi-quantitatively analyze the expression of HSP70 mRNA in the ipsilateral cerebral hemisphere in a neonatal rat model of hypoxia-ischemia. Groups were divided into those pre-treated with DEX prior to hypoxia-ischemia, those treated with DEX immediately following hypoxia-ischemia, those pre-treated with DEX prior to sham, and normal controls, respectively. Results　Cerebral HI increased HSP70 mRNA expression. The peak time of overexpression was 12 h after HI(3.868±1.421), and maintained at a higher level during 12～48 h(3.186±0.803, 2.568±0.709). The intensity of HSP70 mRNA expression was greatly decreased or almost abolished completely by the pretreatment with DEX. At 12 h, 24 h and 48 h after this treatment, HSP 70 mRNA expression were 0.526±0.593, 0.492±0