MdNAC52调控苹果花青苷和原花青素生物合成的机理.pdfVIP

符号中英文缩略说明 Amp: Ampicillin, 氨苄青霉素 ACE: Acetosyringone, 乙酰丁香酮 bp: Base pair, 碱基对 cDNA: Complementary DNA, 互补 DNA CDS: Coding domain sequence, 基因编码序列 Cb: Carbenicillin, 羧苄青霉素 DNA: Deoxyribonucleic acid, 脱氧核糖核酸 DMSO: Dimethyl sulphoxide, 二甲基亚砜 DMACA: 4-Dimethylaminocinnamaldehyde ，4-二甲氨基肉桂醛 ddH O: Distilled and deionized water, 重蒸水 2 dNTP: Deoxy ribonucleoside triphosphate, 脱氧核糖核苷三磷酸 E.coli : Escherichia coli, 大肠杆菌 EDTA: Ethylene diaminetetraacetic acid, 乙二胺四乙酸 IPTG: Isopropylthio-β-D-galactoside, 异丙基-β-D-硫代半乳糖苷 IAA: Indole-3-acetic acid, 吲哚乙酸 Kan: Kanamycin, 卡那霉素 LB: luria-bertani medium LB, 培养基 MS: Murashige and Skoog media MS, 培养基 MES: 2-morpholinoethanesulfonic acid, 2- 吗啉乙磺酸 NAA: Naphthalene acetic acid, 萘乙酸 OD: Optical density, 光密度 PCR: Polymerase chain reaction, 聚合酶链式反应 PBS: Phosphate buffer solution, 磷酸缓冲液 PVDF: Polyvinylidene fluoride, 聚偏二氟乙烯膜 PMSF: Phenylmethanesulfonyl fluoride, 苯甲基磺酰氟 pH: Pondus hydrogenii, 氢氧离子浓度指数 PA: Proanthocyanidin, 原花青素 RT-PCR: Reverse transcriptase-polymerase chain reaction, 反转录酶-聚合酶链式反应 rpm: Revolutions per minute, 转/分钟 SDS: Sodium dodecyl sulfate, 十二烷基硫酸钠 TDZ: Thidiazuron, 噻苯隆 TEMED: N,N,N,N-Tetramethylethylenediamine, 四甲基乙二胺 TE: Tris·Cl, EDTA buffer, Tris·Cl 、EDTA 缓冲液 TF: Transcription factor, 转录因子 Tris: Tris (hydryxymethy) laminomethane, 三羟甲基氨基甲烷 X-gal: 5-Bromo-4-chloro-3-indolyl-β-Dgalactoside, 5-溴-4-氯-3-吲哚-β-D-半乳糖苷 YEP: Yeast extract and peptone medium, YEP 培养基 2,4-D: 2, 4-Dichlorophenoxyacetic acid, 2,4-二氯苯氧乙酸 3-AT: 3-amino-1,2,4-triazole, 3-氨基 -1,2,4 -三氮唑 6-BA: N-(Phenylmethyl)-9H-purin-6-amine, 6-苄氨基嘌呤 目 录 中文摘要 ······························································································ Ⅰ 英文摘