食品中有毒有害物质的分析.pptVIP

2019 年 2 月 21 日 Choose View, Header and Footer to enter text here 31 Experimental To demonstrate ProSep Function in Large Volume Injection using a simple 4 component mixture. 目的 The ProSep preseparation column was phase coated with HT-5, contained HT-5 coated fibers and a plug of silica wool in the upper 4-7 cm of the column. 6890 Plus GC with ALS made all injections onto 30m, .25 mm id, .25 um film HP- 5 MS column …. MSD was operated in full scan, …. 31 2019 年 2 月 21 日 Choose View, Header and Footer to enter text here 32 LVI （大体积进样）的优势 LVI allows greater flexibility by providing the choice of – Lowered detection limits with an existing extraction methodology OR Less Sample Prep / Extraction while maintaining required detection limits ProSep has applications demonstrating both approaches and others (e.g., derivatization “during injection”, …) 32 2019 年 2 月 21 日 Choose View, Header and Footer to enter text here 33 LVI 和化学电离质谱 = 提高选择性和灵敏度 20 attograms / ul of Octafluoronaphthalene acquired using LVI with ECNI – MS on 5973 (44,000 molecules / ul ) 33 2019 年 2 月 21 日 Choose View, Header and Footer to enter text here 34 PCI+SIM +LVI 技术分析 NDMA 20 fg/uL 200 fg/uL PCI-NH 3 -SIM chromatograms of 50 uL injections of NDMA standards in CH 2 Cl 2 . Applying a concentration recovery factor of 500, 200 fg/ul = 0.4ng/l (0.4 ppt) 20 fg/ul = 0.04ng/l (40 ppq) 34 2019 年 2 月 21 日 Choose View, Header and Footer to enter text here 35 NDMA 分析的线性及重现性 NDMA quantitation curve from 20 fg/ul to 4000 fg/ul r 2 =0.999 Concentration RSD Ratio 75/92 RSD Response (Area) 20 2.9% 2.4% 40 2.2% 3.2% 200 0.7% 0.8% 2000 0.7% 1.7% 4000 0.3% 0.9% Reproducibility in 75/92 m/z ratio and response (n=5) for concentrations of NDMA from 20 to 4000 fg/ul 35 2019 年 2 月 21 日 Choose View, Header and Footer to enter text here 36 结论 Apex ProSep Preseparation Inlet ? Behaves as a Chromatographi