病理学：肿瘤（四）.ppt

* * * * * * * * * * * * Visualization of the interaction of host dendritic cells and tumor cells in fresh tumor tissue.Manyhost-derived GFP-expressing dendritic cells directly contacting B16F10-RFP melanoma cells with their dendrites (arrows) are visualized. Dendritic cell–lymphocyte clusters can be seen in certain regions of the image (arrowheads) 3 weeks after tumor implantation. Visualization of extensive host lymphocyte infiltration in fresh tumor tissue. Numerous host-derived GFP-expressing immune cells, mainly lymphocytes, are infiltrating into the tumor mass and forming immune clusters (arrowheads) in orthotopically implanted MTT060562-RFP mouse breast cancer by day 3 after tumor implantation. Visualization of angiogenesis in live tumor tissue 3 weeks after s.c. injection of B16F10-RFP melanoma cells in the transgenic GFP mouse. (A) Visualization of angiogenesis onset and development imaged in live tumor tissue. Host-derived GFP-expressing fibroblast cells (arrows) and endothelial cells (arrowheads) are shown forming new blood vessels in the RFP-expressing B16F10 melanoma. (B) Well developed, host-derived GFP-expressing blood vessels are visualized in the RFP-expressing mouse melanoma. (C) Tumor vasculature in viable tumor tissue and necrotic tumor tissue in the same tumor mass are visualized. GFP-expressing tumor vasculature can be readily identi-fied in the area where the tumor tissue maintained good viability; however, only remnants of GFP-expressing vasculature can be visualized in the necrotic area. (Scale bars, 50 m.) Whole-body image of orthotopically growing HCT 116-RFP human colon cancer in GFP nude mouse. Image was acquired in a fluorescence light box with a CCD camera 10 weeks after orthotopic implantation ofHCT116-RFP cells. * (A) Host GFP macrophage (arrowhead) contacting RFP cancer cell (arrow). (B) GFP macrophage (arrowhead) engulfing RFP cancer cell (arrow). (C) RFP cancer cell (arrow) engulfed by GFP macrophage (arrowhead). (D) RFP cancer c