早期宫颈癌耐药基因与临床预后关系回顾性研究.docxVIP

早期宫颈癌耐药基因与临床预后关 系回顾性研究 【摘要】目的：探讨耐药基因p糖蛋白、 谷胱甘肽s转移酶、拓扑异构酶n、胸昔酸 合成酶在宫颈癌中的表达及临床意义。 方法: 应用免疫组织化学方法检测 65例宫颈癌中 pgp、TOpN、GSR、TS的表达，并分析其 与临床预后之间的关系。结果:pgp的表达与 肿瘤分化程度、淋巴结转移有关，与 UICC 分期、局部复发无关；TOpcn的表达与淋巴 结转移、局部复发有关，与肿瘤分化程度、 UICC分期无关；GSR的表达与各个指标之 间均存在相关；TS与淋巴结转移、UICC分 期有关，与局部复发、肿瘤分化无关。经多 因素cox比例风险模型分析显示：局部复发、 淋巴结转移及TOP6、GSR的表达与宫颈 癌患者术后生存率有关。结论：宫颈癌中pgp、 TOpE、GSR、TS的联合检测对判断宫颈 癌的预后十分必要；局部复发、淋巴结转移 及TOpE、GSR的表达可作为宫颈癌患者 独立的预后因素。 【关键词】宫颈癌；多药耐药；免疫组织 化学；预后 【ABSTRACT Objective:To investigate the expression of pglycoprotein(pgp) 、 glutathionestransferase 兀(GST兀)、 topoisomerase H (TOpoll) and thymidylate synthase(TS)and their clinical significance in Cervical :The expressions of pgp 、 TOpS、GSR、TS proteins were detected by immmunohistochemistry method in sixtyfive patients with Cervical Carcinoma who were followed up more than ten relationship between the expression of antidrug proteins and clinical prognosis were :The expression of pgp was associated with lymph node metastasis and histological grading (P005) , but not related with partial recurrence and UICC staging(P005); The expression of TOpS was associated with lymph node metastasis and partial recurrence (P005) , and not associated with histological grading and UICC staging(P005);The expression of GSR was associated with all parameters (P005); The expression of TS was associated with lymph node metastasis and UICC grading (P005) , but had no relationship with partial recurrence and histological staging(P005); After multivariate Cox analysis, survival rate was correlated with partial recurrence, lymph node metastasis and expressions of TOPO H and GST 兀(P005) and for the operated patierts. Conclution:The detection of the drug resistance genes: TOpo H and GST 兀 has important clinical significence for the selection of chemotherapy regimen ; partial recurrence, lymph node metastasis and the expression of GSR and TOpoll can be regarded as independent prognostic factors in cervical carcinoma. 【KEY WORD SCervical carcinoma;Multidrug resista