生殖生物学理论与相关技术：胚胎干细胞.pptVIP

Patient iPSCs could be induced into germ cell lineage differentiation Premeiotic Germ Cells BMPs RA Spontaneous Differentiation Meiosis R115866+FRSK+LIF+FGF2 Meiotic Germ Cells hESCs or hiPSCs DAZ+DAZL+BOULE in vitro The expression of Leydig and Sertoli Cell markers in the Spontaneous Differentiation Leydig Cells Sertoli Cells X chromosome inactivation H3K27me3 MacroH2A1 KS-fs KS-iPSC-1 KS-iPSC-4 KS-iPSC-8 KS-iPSC-11 Dysregulation of X Linked Genes Up-regulated Genes (fc=2) Down-regulated Genes (fc=2) XCI is Possibly Incomplete Location No. of Genes Up-regulation (31) X Chromosome 11 Y Chromosome 1 Autosomes 19 Down-regulation (9) X Chromosome 0 Y Chromosome 0 Autosomes 9 Gene Ontology Analysis of the Differentially Expressed Genes between XXY Cells and XY Cells P Value 0.5, fc=1.5 Potential applications of KS patient iPS cells Study the mechanisms of development of clinical symptoms in KS patients; Explore the possibility of using KS iPS cells to differentiate into normal germ cells for the treatment Dysregulation of X linked genes Up-regulated Genes (fc=2) Down-regulated Genes (fc=2) XCI is possibly incomplete Location No. of Genes Up-regulation (31) X Chromosome 11 Y Chromosome 1 Autosomes 19 Down-regulation (9) X Chromosome 0 Y Chromosome 0 Autosomes 9 Gene ontology analysis of the differentially expressed genes between XXY cells and XY cells P Value 0.5, fc=1.5 * * * three routes: directly from pre-implantation embryos, through reprogramming of somatic cells, and through reversion of primed pluripotent cells. * Preferential activation of DE * Allowed by ethic? * 体细胞重编程广义上可以定义为分化的体细胞通过某些特定的方法改变其细胞命运使其转化形成另外一种细胞类型的过程。可分为两种情况，第一种情况是将体细胞重编程到多能干细胞的状态，这个过程又叫做体细胞脱分化，一定程度上可以认为是多能干细胞分化事件的逆过程。第二种情况是将体细胞直接重编程转分化形成其他类型的体细胞。狭义上体细胞重编程就特指第一种情况，本课题中我们研究和讨论的也主要是这种情况。 * By groups of 1. James Thomson published in Science (Oct4, Sox2, Nanog and Lin28) with lentivirus 2. Shinya Yamanaka published in Nature Biotec. (Oct4, Sox2, Klf4 and c-Myc) with