生殖生物学理论与相关技术：05、着床过程和机理.pptVIP

Regulations of Angiogenesis VEGF (vascular endothelial growth factor) PDGF (platelet-derived growth factor) PAF (platelet-activating factor) inhibitors ECM降解-为胚泡入侵和胎盘形成提供条件 纤连蛋白、玻连蛋白、Ⅲ、Ⅴ、Ⅵ型胶原等降解 基质粘性下降，间质水肿 MMPs(2、9) （金属蛋白酶）／MMPs组织抑制因子-1的平衡 原蛋白酶5／6是MMP的激活剂之一 Horse-PC6 MMP9 Monkey-PC6 Human-PC6 IL-1，6，8，11、P/E, LIF, hCG, O2 表皮生长因子家族（Hb-EGF、TGF-α、TGF-β、EGF); NGF, IGF, TNF, GmCGF; LIF, VEGF... 整合素家族（α1β1、αvβ3、α4β1）、粘附分子、凝集素 , Proteanases, Proproteinases... ? Implantation involves a complex network of molecular signaling  Leukaemia inhibitory factor (LIF) -at the top of molecular network LIF and LIF-R : expressed in pre-implantation embryo Secreted from the uterus Lacking a functional LIF gene -their blastocysts fail to implant Maximal expression of LIF is during the implantation window leading molecule Involved in immune tolerance Heparin-binding EGF-like growth factor Expressed in stromal and epithelial cells of the uterus Regulate endometrial proliferation, secretion ,decidualization Involved in the regulation of blastocyst implantation Epidermal Growth Factor Expressed both in decidual and trophoblastic cells Induces trophoblast invasion trophoblast differentiation trophoblast proliferation Regarded a major regulator of the implantation process Insulin-like growth factor binding protein-1 Main secretory product of the decidualized endometrium Increase the lytic activity of trophopblasts Increase trophoblast migration Transforming growth factor β Expressed both in endometrial and trophoblastic cells Inhibit trophoblast proliferation and invasion Stimulating TIMP secretion Decreasing MMP activation Human Chorionic Gonadotropin(hCG) Syncytiotrophoblastic cells secreted Coincide with trophoblast development Endometrial/endothelial LH/hCG-R Maintenance of the corpus luteum Increase trophoblas