蛋白质相互作用的生物信息学 .pptxVIP

蛋白质相互作用的生物信息学高友鹤中国医学科学院 基础医学研究所蛋白质相互作用的生物信息学实验数据蛋白质相互作用数据库高通量实验数据的验证蛋白质相互作用网络计算预测蛋白质相互作用实验数据蛋白质相互作用的知识来源于实验。高通量地应用传统实验方法获取大量相互作用信息。高通量的数据需要验证。高通量实验方法Curr Opin Struct Biol 2003,13:377Yeast two-hybrid assay Benefits: in vivo. Don’t need pure proteins. Don’t need Ab. Drawbacks: only two proteins are tested at a time (no cooperative binding); it takes place in the nucleus, so many proteins are not in their native compartment; and it predicts possible interactions, but is unrelated to the physiological setting. Mass spectrometry of purified complexes Benefits: several members of a complex can be tagged, giving an internal check for consistency; and it detects real complexes in physiological settings. Drawbacks: it might miss some complexes that are not present under the given conditions; tagging may disturb complex formation; and loosely associated components may be washed off during purification.Correlated mRNA expression Benefits:it is an in vivo technique, albeit an indirect one; and it has much broader coverage of cellular conditions than other methods.Drawbacks: it is a powerful method for discriminating cell states or disease outcomes, but is a relatively inaccurate predictor of direct physical interaction; and it is very sensitive to parameter choices and clustering methods during analysis.9、我们的市场行为主要的导向因素，第一个是市场需求的导向，第二个是技术进步的导向，第三大导向是竞争对手的行为导向。10、市场销售中最重要的字就是“问”。11、现今，每个人都在谈论着创意，坦白讲，我害怕我们会假创意之名犯下一切过失。12、在购买时，你可以用任何语言；但在销售时，你必须使用购买者的语言。13、He who seize the right moment, is the right man.谁把握机遇，谁就心想事成。14、市场营销观念：目标市场，顾客需求，协调市场营销，通过满足消费者需求来创造利润。15、我就像一个厨师，喜欢品尝食物。如果不好吃，我就不要它。16、我总是站在顾客的角度看待即将推出的产品或服务，因为我就是顾客。17、利人为利已的根基，市场营销上老是为自己着想，而不顾及到他人，他人也不会顾及你。Genetic interactions (synthetic lethality). Benefits: it is an in vivo technique, albeit an indirect one; and it is amenable to unbiased genome-wide screens.Drawbacks: not necessarily physical interactions蛋白质相互作用的生物信息学实验数据蛋白质相互作用数据库高通量实验数据的验证蛋白质相互作用网络计算预测蛋白质相互作用蛋白质相互作用数据库Curr Opin Struct Biol 2003,13:377THE DIP DATABASEData