贺晓杰生化论文.docVIP

齐齐哈尔大学 毕业设计（论文） 题 n低剂量微波辐射对重金属污染下玉米幼苗生长发育的影响 学 院 生命科学与农林学院 专业班级 生物技术083班 学生姓名 贺晓杰 指导教师 吴旭红教授 成 绩 2012 年 6 月 20 口 摘要 对浸泡10h后的种子分别釆取Os, 5s, 10s, 15s, 20s的低火微波处理(微波强度为 180mW/cm2) o用盆栽的方法培养玉米幼苗，7d后测定发芽率，lid后，测发芽指数和 活力指数。待玉米长至三叶期后，每天早晚用注射器滴灌5 mL CdCl?溶液，溶液设低、 中、高3个浓度梯度,即0.01, 0.1, 1.0 mmol/L,对照组(CK)浇等量自来水,3 d后釆 集玉米叶片测定生理指标。与对照组相比较，低剂量辐射可以提高玉米种子发芽率，高 剂量辐射降低发芽率，4种不同时间长度的处理均可以提高蛋白酶的活性，经过高浓度 CdCl?处理的玉米幼苗叶片屮的超氧化物歧化酶(SOD)和过氧化物酶(POD)等酶的活性 低于对照组，蛋白质的含量低于对照组，叶绿素a、b的含量明显低于对照。这表明，用 适宜剂量的微波辐射处理玉米种子能提高玉米的发芽率，有助于玉米幼苗的生长发育， 低浓度的镉溶液能够促进玉米幼苗中S0I)和P0I)的活性，高浓度的镉溶液对其产生抑制作 用。对叶绿素的影响则表现为低浓度的镉对叶绿素a和叶绿素b均有刺激作用，高浓度的 镉对其则起到了抑制作用。 关键词：玉米幼苗；微波辐射；氯化镉；发芽指数；蛋口质含量；幼苗发育 Abstract Soak the seeds after lOh, respectively, to take the Os, 5s, 10s, 15s, 20s, the low-fire microwave treatment (microwave intensity of 180mW/c m2). Pot method to cultivate maize seedlings, measured after germination rate 7d, lid, measured germination index and vigor index. Corn up to three-leaf stage until after day, morning and evening drip irrigation with a syringe 5 mL of CdCh solution, the solution set low, medium and high concentration gradient 3, namely 0.01,0.1,1.0 mmol / L, control group (CK), pouring the same amount tap water, collected 3 d after corn leaves determination of physiological indicators. Compared with the control group, low doses of radiation can be improved maize seed germination rate, high-dose radiation to reduce the germination rate, four different length of time processing can improve the activity of the protease, superoxide after the the CdCh processing of the high concentration of leaves of maize seedlings the enzyme superoxide dismutase (SOD) and peroxidase (POD) activity was lower than in the control group, the protein content is lower than the control group, chlorophyll a and b were significantly lower than the control. This suggests that appropriate doses of microwave radiation processing of com seedlings can improve corn germination rate, contribute to the growth and development of maize seedl