半胱氨酸蛋白酶抑制剂对离体大鼠海马脑片CA1区神经元低氧性损伤的影响医学.docVIP

半胱氨酸蛋白酶抑制剂对离体大鼠海马脑片CA1区神经元低氧性损伤的影响医学 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：半胱氨酸蛋白酶抑制剂对离体大鼠海马脑片CA1区神经元低氧性损伤的影响医学 1 1 材料与方法 3 2 结果 4 3 讨论 5 文2：苦参碱对大鼠急性心肌缺血性损伤的保护作用 6 1 材料和方法 7 2 结果 8 3 讨论 9 参考文摘引言： 10 原创性声明（模板） 11 文章致谢（模板） 12 正文 半胱氨酸蛋白酶抑制剂对离体大鼠海马脑片CA1区神经元低氧性损伤的影响医学 文1：半胱氨酸蛋白酶抑制剂对离体大鼠海马脑片CA1区神经元低氧性损伤的影响医学 ［ABSTRACT］ObjectiveTo investigate the effects of cysteine protease inhibitor PD150606 on hypoxic damage to neuro in CA1 region of hippocampus in rats. MethodsThe hippocampus slices were randomly divided into Con （ n =6）， PD150606 （PD50， n =10） and DMSO （ n =8） groups. The slices in Con group were treated with aCSF at 37 ℃ for 60 min before intracellular recording， and the slices in PD and DMSO groups were incubated with 50 μmol/L PD150606 and DMSO for 60 min before in-tracellular recording. All slices in each group were subjected to 5 min hypoxia and followed by 60 min of re-oxygenation. At the end of electrophysiological research， the slices were stained with propidium idodide （PI） and the PI positive neuro in CA1 region was counted. ResultsPD150606 significantly delayed rapid depolarization and inhibited rapid depolarization （ P ）. After 60min of re-oxygenation， eight out of 10 neuro in the PD group had its functional recovery. The dead neuro in PD group were significantly less than those in Con and DMSO groups （ P ）. ConclusionCalpai inhibitor PD150606 could significantly inhibit the hypoxic damage to neuro and enhance the electrophysiological functional recovery of neuro in CA1 region of hippo-campus in rats. ［KEY WORDS］cysteine proteinase; hippocampus;hypoxia-ischemia， brain 半胱氨酸蛋白酶（Calpai）是广泛存在于哺乳类动物细胞浆内的半胱氨酸蛋白酶家族，依赖细胞浆内的钙离子浓度增加而激活。激活后的Calpai可以降解细胞骨架蛋白、电压及配体门控性离子通道及与信号转导有关的G蛋白及酶等［1，2］。应用形 态学及生化等指标的研究显示，Calpai抑制剂可以显著减轻神经元缺血低氧性损伤［3］。然而，该酶抑制剂能否促进缺血低氧后神经元功能的恢复还缺乏直接的证据。本实验采用大鼠离体海马脑片，运用细胞内记录技术，结合碘化丙啶（PI）染色法，计数海马CA1区死亡神经元，以期对Calpai抑制剂PD150606对神经元低氧性损伤的影响进行客观及全面的评价。 1 材料与方法 材料 选择SD大鼠12只，雄性，体质量280～340 g，由美国纽约州立大学医学中心提供。人工脑脊液（aCSF），含有N