三七皂苷对人骨髓造血细胞凋亡相关蛋白表达的影响.docVIP

三七皂苷对人骨髓造血细胞凋亡相关蛋白表达的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：三七皂苷对人骨髓造血细胞凋亡相关蛋白表达的影响 1 三七皂苷注射液半固体造血祖细胞集落培养及P刺激试验 3 文2：神经生长因子对新生大鼠HIBD后海马区凋亡相关蛋白表达的影响医学 8 1材料和方法 9 参考文摘引言： 13 原创性声明（模板） 14 文章致谢（模板） 15 正文 三七皂苷对人骨髓造血细胞凋亡相关蛋白表达的影响 文1：三七皂苷对人骨髓造血细胞凋亡相关蛋白表达的影响 Expression of Apoptosis-related Protei in the Human Bone Marrow Hematopoietic Cells Treated by Panax Notoginosides Abstract The study was aimed to investigate the action of Panax Notoginosides (P，extracted from notogieng herb) on the expression of the apoptosis-related protei (Daxx，Fas) and tracription facto (NFkB、c-Rel) in the hematopoietic cells and to explore the mechanisms of supporting cells to colony formation of CFU-GM and CFU-E in human bone marrow was assayed in the presence of various concentratio of viability of cells was assayed by trypan blue and the changes of cell morphology were observed with Annexin-V positive cells were detected by lineages of human myeloid HL-60，erythroid K562，megakaryoid CHRF-288 and Meg-01 cells were incubated in addition of P（10 mg/L）for 14 nuclear or cytoplasm protein of cells was extracted and analyzed by Western blot with monoclonal antibodies agait Daxx，Fas or NFkB， results showed: (1)the proliferation on hematopoietic progenitor cells （CFU-GM and CFU-E） and four cell lines was promoted by P; (2) after the four cell lines were promoted by P and hungered through wiping off the sera，the viability of the four cell lines was high without significant morphological change and neither the detection of Annexin-V positive cells; (3) the expression of Daxx and Fas protein could be inhibited by Blot showed that Daxx in four cell lines treated by P were % lower than that in untreated Fas protein was also descended in three cell lines of K562，CHRF-288 and Meg-01 by % respectively，while Fas protein in HL-60 cells was no detectable difference after P treatment.(4) The tracription facto NFkB and c-Rel protein could be increased by NFkB、c-Rel protein were also