糖尿病胃肠组织微血管中细胞外信号调节激酶磷酸化的探讨.docVIP

糖尿病胃肠组织微血管中细胞外信号调节激酶磷酸化的探讨 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：糖尿病胃肠组织微血管中细胞外信号调节激酶磷酸化的探讨 1 1 材料和方法 3 1．4 免疫组织化学染色 4 2 结果 4 2．1 组织病理学观察 4 2．2 免疫组化结果 5 3 讨论 6 文2：细胞外信号调节激酶在局灶性脑缺血中的表达 7 1材料和方法 8 参考文摘引言： 14 原创性声明（模板） 15 文章致谢（模板） 16 正文 糖尿病胃肠组织微血管中细胞外信号调节激酶磷酸化的探讨 文1：糖尿病胃肠组织微血管中细胞外信号调节激酶磷酸化的探讨 Abstract: Objective To explore the mechanism of diabetic microangiopathy. Methods The phosphorylation of extracellular signal-regulated kinase(ERK1/2) and ets like gene-1 (ELK-1)in the gastrointestinal microvascular endothelial cells and smooth muscle cells in diabetic with non-diabetic patients were observed with immunohistochemistry. Results phospho-P44/P42MAPK positive expression in microvascular endothelial cells, the positive rate of Gastrointestinal organizatio laye in diabetic group were significantly higher than that in non-diabetic group(P＜)；Phosphorylation-ELK-1 positive expression in microvascular endothelial cells, the positive rate of gastrointestinal mucosa, submucosa, muscularis and serosal layer microvascular endothelial cells in diabetic group were %, %, % and %, and in non-diabetic group (respectively %, %, % and 0). in addition to serosal layer, the remainiy laye were no significant differenles(P＞). In diabetic group, phospho-P44/P42MAPK positive expression rate of gastrointestinal mucosa, submucosa, muscularis, serosal layer microvascular smooth muscle cells had no significant difference compared with the non-diabetic group (P＞)；In the diabetic group, Phosphorylation-ELK-1 positive expression rate of gastrointestinal mucosa, submucosa, muscularis, serosal layer microvascular smooth muscle cells had significant difference compared with the non-diabetic group (P＜). In microvascular endothelial cells , phospho-P44/P42MAPK and Phosphorylation-ELK-1 expression showed a positive correlation (r =, P= )；In microvascular smooth muscle cells, phospho-P44/P42MAPK and Phosphorylation-Elk-1 expression showed a positive correlation (r=,