赛莱西布对肝癌HepG2细胞增殖与凋亡影响.docVIP

赛莱西布对肝癌HepG2细胞增殖与凋亡影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：赛莱西布对肝癌HepG2细胞增殖与凋亡影响 1 1 材料与方法 3 11 材料 3 12 方法 3 13 统计分析 应用SPSS100软件进行t检验。 6 2 结果 6 1：25μmol/L；2：空白对照；3：50μmol/L 6 3 讨论 7 文2：赛莱西布对肝癌HepG2细胞增殖与凋亡影响 8 1材料与方法 10 11材料 10 12方法 10 123dna梯度电泳法检测hepg2细胞凋亡dna片断 11 13统计分析应用spss100软件进行t检验。 12 1：25μmol/l；2：空白对照；3：50μmol/l 13 参考文摘引言： 15 原创性声明（模板） 16 文章致谢（模板） 17 正文 赛莱西布对肝癌HepG2细胞增殖与凋亡影响 文1：赛莱西布对肝癌HepG2细胞增殖与凋亡影响 Effects of cyclooxygenase-2 on proliferation and apoptosis in HepG2 cells Abstract： Objective To examine the effects of a selective inhibitor of cox-2,celecoxib on growth and apoptosis in HepG2 HepG2 cells were treated with various concentratio of growth was measured by MTT,apoptosis was detected by DNA fragmentation assay,and cox-2 activity was measured by RIA;Expression of caspase-3 protein in HepG2 cells was determined by western blot,and caspase-3 activity was measured using colorimetric After HepG2 cells were treated with different concentratio of celecoxib (,25,50μmol/L),celecoxib could significantly suppress the growth of HepG2 cells respectively compared with the control group (P),and celecoxib-treated HepG2 cells produced a distinct oligosomal ladder,the characteristics of cells undergoing apoptosis;Meanwhile,The caspas-3 expression and activity of celecoxib treated HepG2 cells were increased respectively as compared with the control groups(P).Furthermore,the level of cox-2 activity was reduced in celecoxib-treated HepG2 cells respectively compared with the control group (P).Conclusion Celecoxib could inhibit the proliferation and increase apoptosis in HepG2 cells by cox-2 and caspase3 pathways. Key words： HepG2 cell;cyclooxygenase-2;celeeoxib;proliferation;apoptosis 近年来的研究表明，环氧合酶-2(cox-2)在结直肠癌、食管癌、肺癌等多种人类肿瘤组织中高表达〔1-3〕。最新报道显示〔4，5〕，cox-2在肝癌组织中也高表达，并且在肝硬化组织中过表达，提示cox-2可能是肿瘤防治的一个新靶点〔3〕。非甾体类抗炎药(noteroidal anti-inflammatory drugs，AIDs)具有预防和辅助 治疗 肿瘤尤其胃肠道肿瘤的作用〔6〕，但AIDs抑制肿瘤的作用机制尚不明确。本研究选择AIDs类药物中的1种高选择