内皮型一氧化氮合酶基因多态性与冠心病关系.docVIP

内皮型一氧化氮合酶基因多态性与冠心病关系 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：内皮型一氧化氮合酶基因多态性与冠心病关系 1 1 对象与方法 3 12 方法 3 2 结果 4 3 讨论 6 文2：内皮型一氧化氮合酶基因多态性与冠心病关系 6 1对象与方法 8 12方法 8 122基因组 8 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 13 正文 内皮型一氧化氮合酶基因多态性与冠心病关系 文1：内皮型一氧化氮合酶基因多态性与冠心病关系 Association between polymorphism of endothelial nitric oxide synthase gene and coronary heart disease Abstract： Objective To assess the association between intron 4 VNTR polymorphism in endothelial nitric oxide synthase (eNOS) gene and premature coronary heart disease(p-CHD).Methods A hospital-based case-control study was CHD patients were recruited as this study hurdred and eighty-eight CHD patients diagnosed at/before 55 yea old for males and 65 for females were assigned to p-CHD case group with other 315 CHD patients as the control were detected by polymorphism chain reaction (PCR) The genotype frequencies of c/c,c/b,b/b,b/a,a/a were 053%,000%,8138%,1649%,160% in p-CHD group respectively and 000%,032%,7746%,2032%,190% in the control group was no significant difference between the p-CHD and the control group for comparing genotype frequencies of c/c+c/b+b/b with b/a+a/a (P=0268,OR =1294,95%CI=0820～2043).The allele frequencies of c,b,a were 053%,8963%,984% in the p-CHD group respectively and 016%,8778%,1206% in the control group was also no significant difference between the p-CHD and the control group for comparing allele frequencies of c+b with a (P=0280，OR=1257，95%CI=0830～1904).Stepwise multiple logistic regression analysis at 005 significant level with adjust ment of sex,smoking,alcohol drinking,systolic blood pressure,overweight,waist-hip ratio,serum triglyceride,serum total cholesterol covariates showed that the eNOS4a had no significant effect on p-CHD (P=0427,OR=0819,95%CI=0500～1341).Conclusion eNOS intron 4 a/b VNTR polymorphism is not a risk factor for p-CHD. Key words： coronary heart disease;nitric oxide synthase;endothelial;gene 遗传因素在冠状动脉粥样硬化性心脏病(冠心病CHD)发病中具有重要作用，但