牛磺酸对大鼠视网膜光化学损伤c.docVIP

牛磺酸对大鼠视网膜光化学损伤c 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：牛磺酸对大鼠视网膜光化学损伤c 1 1 材料与方法 3 15 统计分析 采用SPSS100统计软件进行方差分析。 4 2 结果 4 22 牛磺酸对光照后大鼠视网膜cfos转录以及表达的影响 4 3 讨论 5 文2：牛磺酸对大鼠视网膜光化学损伤c 6 1材料与方法 7 15统计分析采用spss100统计软件进行方差分析。 8 22牛磺酸对光照后大鼠视网膜cfos转录以及表达的影响 9 参考文摘引言： 10 原创性声明（模板） 12 文章致谢（模板） 12 正文 牛磺酸对大鼠视网膜光化学损伤c 文1：牛磺酸对大鼠视网膜光化学损伤c Effects of taurine on cfos expression of rat retina after photochemical damage Abstract： Objective To observe the effect of dietary supplementation with taurine on photoreceptor apoptosis and further investigate changes of cfos expression of rat retina after photochemical Seventy rats were randomly divided into control group (Control) and 4% taurine supplementation group (Taurine).The subjects were exposed to (3000±200) lx tramitted by six cold white lights for 0,1,3,6,9,12,24 apoptotic photoreceptor cells were detected using TUNEL method;Total RNA and protein of retina were extracted;relative cfos mRNA levels were determined using RT-PCR and cfos protein levels were detected by western-blot With the light exposure time prolonged,apoptosis index (AI) of photoreceptor cells was elevated,among which the AI of 12h were (±)%and (±) % in Taurine and control group(P).The cfos mRNA of rat retina was traiently induced after one-hour light exposure and it was lower in taurine group (P);cfos protein level increased after three hour exposure and it was lower in taurine group (P).Conclusion Taurine can partly reduce the tracription and tralation of c-fos,which would further inhibit the apoptosis signal traduction and protect the rat retina from photochemical damage. Key words： taurine;photochemical damage;apoptotic cell;cfos 视网膜是视觉形成的重要组织结构，若光照时间或光照强度等超过视网膜承受力，则会导致光化学损伤〔1〕，损伤早期视功能减退，光感受器细胞丢失，后期内层神经元变性坏死，最终导致失明〔12〕。近年研究认为，凋亡是视网膜光化学损伤中光感受器细胞丢失的主要机制〔1,3〕，持续高强度光照使视网膜内自由基和脂质过氧化产物激增，同时氧化应激基因AP1表达上调促进凋亡信号转导，且证实其亚单位cfos是必需调控因子。牛磺酸与视网膜生长发育和功能维持关系密切，前期实验已证实，4％牛磺酸能有效保护光化学损伤条件下的大鼠视网膜〔4〕。本研究拟进一步观察其对光感受器细胞凋亡的影响，并从cfos表达变化角度探讨防护机制。 1